| KOŠŤÁL, Vratislav, Marta ZEISBERGEROVÁ, Zuzana HROTEKOVÁ, Karel ŠLAIS a Vladislav KAHLE. Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids. Electrophoresis. 2006, roč. 27, č. 23, s. 4658-4665. ISSN 0173-0835. |
Další formáty:
BibTeX
LaTeX
RIS
@article{747070,
author = {Košťál, Vratislav and Zeisbergerová, Marta and Hroteková, Zuzana and Šlais, Karel and Kahle, Vladislav and Glatz, Zdeněk},
article_number = {23},
keywords = {amino acids; CE; fluorescence detection; liquid core waveguide; separation methods},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids},
volume = {27},
year = {2006}
}
TY - JOUR
ID - 747070
AU - Košťál, Vratislav - Zeisbergerová, Marta - Hroteková, Zuzana - Šlais, Karel - Kahle, Vladislav - Glatz, Zdeněk
PY - 2006
TI - Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids
JF - Electrophoresis
VL - 27
IS - 23
SP - 4658-4665
EP - 4658-4665
SN - 01730835
KW - amino acids
KW - CE
KW - fluorescence detection
KW - liquid core waveguide
KW - separation methods
N2 - A miniaturized post-column fluorimetric detection cell for capillary separation methods based on optical fibers and liquid core waveguides (LCWs) is described. The main part of the detection cell is a fused-silica capillary coated with Teflon AF serving as an LCW. The optical fibers are used both for coupling the excitation source with the detection domain in the LCW and for the axial fluorescence collection from the LCW end. The latter fiber is connected with a compact CCD spectrometer that serves for the rejection of the scattered excitation light and for the fluorescence signal detection. The proposed design offers a compact fluorescence detector for various microcolumn separation techniques without optical elements such as filters or objectives. Moreover, its construction and optical adjustment are very simple and the whole system is highly miniaturized. The function of the detection cell is demonstrated by CE of amino acids labelled by fluorescein-based tags. Separations of different standard amino acid mixtures and plasma samples are presented. The comparison of plasma amino acid levels of individuals being in good health with those of patients with inherited metabolic disorders is also shown.
ER -
KOŠŤÁL, Vratislav, Marta ZEISBERGEROVÁ, Zuzana HROTEKOVÁ, Karel ŠLAIS a Vladislav KAHLE. Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids. \textit{Electrophoresis}. 2006, roč.~27, č.~23, s.~4658-4665. ISSN~0173-0835.
|
