Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero.

KŘÍŽ, Vítězslav, Jaroslav MAREŠ, Parri WENTZEL, Nina FUNA, Gabriela ČALOUNOVÁ, Xiao-Qun ZHANG, Karin FORSBERG-NILSSON, Maud FORSBERG a Michael WELSH. Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero. Developmental dynamics. United States: Wiley-Liss, 2007, roč. 236, č. 9, s. 2485-2492. ISSN 1058-8388. Dostupné z: https://dx.doi.org/10.1002/dvdy.21257.

Základní údaje

Originální název

Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero.

Název česky

Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero.

Autoři

KŘÍŽ, Vítězslav (203 Česká republika, garant, domácí), Jaroslav MAREŠ (203 Česká republika), Parri WENTZEL (752 Švédsko), Nina FUNA (752 Švédsko), Gabriela ČALOUNOVÁ (203 Česká republika), Xiao-Qun ZHANG (156 Čína), Karin FORSBERG-NILSSON (752 Švédsko), Maud FORSBERG (752 Švédsko) a Michael WELSH (840 Spojené státy)

Vydání

Developmental dynamics, United States, Wiley-Liss, 2007, 1058-8388

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.084

Kód RIV

RIV/00216224:14310/07:00050891

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/dvdy.21257

UT WoS

000249539700012

Klíčová slova anglicky

SHB; knockout; ovulation; malformations; viability in utero; transmission ratio distortion

Štítky

AKR, knockout, malformations, ovulation, rivok, SHB, transmission ratio distortion, viability in utero

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

SHB is an Src homology 2 domain-containing adapter protein that has been found to be involved in numerous cellular responses. We have generated an Shb knockout mouse. No Shb-/- pups or embryos were obtained on the C57Bl6 background, indicating an early defect as a consequence of Shb- gene inactivation on this genetic background. Breeding heterozygotes for Shb gene inactivation (Shb+/-) on a mixed genetic background (FVB/C57Bl6/129Sv) reveals a distorted transmission ratio of the null allele with reduced numbers of Shb+/+ and Shb-/- animals, but increased number of Shb+/- animals. The Shb- allele is associated with various forms of malformations, explaining the relative reduction in the number of Shb-/- offspring. Shb-/- animals that were born were viable, fertile, and showed no obvious defects. However, Shb+/- female mice ovulated preferentially Shb- oocytes explaining the reduced frequency of Shb+/+ mice. Our study suggests a role of SHB during reproduction and development.

Česky

SHB is an Src homology 2 domain-containing adapter protein that has been found to be involved in numerous cellular responses. We have generated an Shb knockout mouse. No Shb-/- pups or embryos were obtained on the C57Bl6 background, indicating an early defect as a consequence of Shb- gene inactivation on this genetic background. Breeding heterozygotes for Shb gene inactivation (Shb+/-) on a mixed genetic background (FVB/C57Bl6/129Sv) reveals a distorted transmission ratio of the null allele with reduced numbers of Shb+/+ and Shb-/- animals, but increased number of Shb+/- animals. The Shb- allele is associated with various forms of malformations, explaining the relative reduction in the number of Shb-/- offspring. Shb-/- animals that were born were viable, fertile, and showed no obvious defects. However, Shb+/- female mice ovulated preferentially Shb- oocytes explaining the reduced frequency of Shb+/+ mice. Our study suggests a role of SHB during reproduction and development.