| LENGEROVÁ, Martina, Zdeněk RÁČIL, Pavlína VOLFOVÁ, Jana LOCHMANOVÁ, Jitka BERKOVCOVÁ, Dana DVOŘÁKOVÁ, Jiří VORLÍČEK and Jiří MAYER. Real-time PCR diagnostics failure caused by nucleotide variability withing exos 4 of human cytomegalovirus major immediate-early gene. Journal of Clinical Microbiology. 2007, vol. 45, No 3, p. 1042-1044. ISSN 0095-1137. |
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@article{749399,
author = {Lengerová, Martina and Ráčil, Zdeněk and Volfová, Pavlína and Lochmanová, Jana and Berkovcová, Jitka and Dvořáková, Dana and Vorlíček, Jiří and Mayer, Jiří},
article_number = {3},
language = {eng},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
title = {Real-time PCR diagnostics failure caused by nucleotide variability withing exos 4 of human cytomegalovirus major immediate-early gene},
volume = {45},
year = {2007}
}
TY - JOUR
ID - 749399
AU - Lengerová, Martina - Ráčil, Zdeněk - Volfová, Pavlína - Lochmanová, Jana - Berkovcová, Jitka - Dvořáková, Dana - Vorlíček, Jiří - Mayer, Jiří
PY - 2007
TI - Real-time PCR diagnostics failure caused by nucleotide variability withing exos 4 of human cytomegalovirus major immediate-early gene
JF - Journal of Clinical Microbiology
VL - 45
IS - 3
SP - 1042-1044
EP - 1042-1044
SN - 00951137
N2 - Here we report how variability in the human cytomegalovirus genome sequence may seriously affect the outcome of its molecular diagnosis. A real-time quantitative PCR assay targeting the major immediate-early gene failed to detect the viral load in some, but not all, clinical samples from hematooncological patients. By amplification and sequencing the DNA across the regions targeted by this assay we found a number of nucleotide substitutions which accounted for decreased primer/probe binding. This decreased the sensitivity of the assay up to 1,000-fold.
ER -
LENGEROVÁ, Martina, Zdeněk RÁČIL, Pavlína VOLFOVÁ, Jana LOCHMANOVÁ, Jitka BERKOVCOVÁ, Dana DVOŘÁKOVÁ, Jiří VORLÍČEK and Jiří MAYER. Real-time PCR diagnostics failure caused by nucleotide variability withing exos 4 of human cytomegalovirus major immediate-early gene. \textit{Journal of Clinical Microbiology}. 2007, vol.~45, No~3, p.~1042-1044. ISSN~0095-1137.
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