Formation of lignans in plant cell culture of Schisandra
chinensis

J 2007

Formation of lignans in plant cell culture of Schisandra chinensis

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Ladislav HAVEL, Irena BOHATCOVÁ, Kateřina CHVÁTALOVÁ et. al.

Základní údaje

Originální název

Formation of lignans in plant cell culture of Schisandra chinensis

Název česky

Tvorba lignanů v rostlinné buněčné kultuře Klanoprašky čínské

Autoři

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Ladislav HAVEL, Irena BOHATCOVÁ, Kateřina CHVÁTALOVÁ a Jiří SLANINA

Vydání

Planta Medica, Stuttgart - New York, Thieme Medical Publishers, 2007, 0032-0943

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.848

Organizační jednotka

Lékařská fakulta

UT WoS

000249395900704

Klíčová slova anglicky

Schisandra; lignans; HPLC

Štítky

HPLC, lignans, Schisandra

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 1. 2011 19:59, Mgr. Jiří Slanina, Ph.D.

Anotace

ORIG CZ

V originále

The fruit of Schisandra chinensis, a woody liana, has been used for centuries in traditional Chinese medicine. The fruit is prescribed for the treatment of hepatitis in China. The active principles are lignans with unusual structures derived from dibenzo[a,c]cyclooctadiene. These lignans have been shown to possess a broad range of biological activities. Recently, it has been found that lignan gamma-schizandin strongly inhibits P-glycoprotein, the overexpression of which is the most frequent cause for cancer multidrug resistance. S. chinensis cultures derived from immature zygotic embryos were developed on Murashige and Skoog and VW5 medium. Embryogenic cultures were established on the medium containing thidiazuron, 2,4-dichlorophenoxyacetic acid and benzylaminopurine. An analytical method based on HPLC-UV was used for the determination of six lignans in plant cell cultures. Our previous results showed that the concentration of lignans in plant cell cultures was mostly lower than that in fruit and leaves. We also detected the lignans in liquid culture media, but the amount of lignans inside the cells was substantially higher. In order to release the lignans stored within the cells, we explored the cultivation of five cell lines with neutral polymeric resin, Amberlite XAD2. We found that the addition of Amberlite XAD2 not only notably enlarged portions of extracellular lignans, but also greatly enhanced the production of lignan deoxyschizandrin, whereas the formation of other lignans was increased only moderately (less than 5fold). Importantly, Amberlite XAD-2 did not reduce the growth of cell cultures. Deoxyschizandrin adsorbed on the polymeric resin can be easily recovered and purified without destruction of the cells.

Česky

Tvorba lignanů v rostlinné buněčné kultuře Klanoprašky čínské byla studována

Návaznosti

GA522/07/0995, projekt VaV

Název: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis

Investor: Grantová agentura ČR, Regulace biosyntézy sekundárních metabolitů v buněčné kultuře Schisandra chinensis

Citovat

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Ladislav HAVEL, Irena BOHATCOVÁ, Kateřina CHVÁTALOVÁ a Jiří SLANINA. Formation of lignans in plant cell culture of Schisandra chinensis. Planta Medica. Stuttgart - New York: Thieme Medical Publishers, 2007, roč. 73, č. 9, s. 1015-1016. ISSN 0032-0943.

@article{750291,
   author = {Březinová, Lenka and Vlašínová, Helena and Havel, Ladislav and Bohatcová, Irena and Chvátalová, Kateřina and Slanina, Jiří},
   article_location = {Stuttgart - New York},
   article_number = {9},
   keywords = {Schisandra; lignans; HPLC},
   language = {eng},
   issn = {0032-0943},
   journal = {Planta Medica},
   title = {Formation of lignans in plant cell culture of Schisandra chinensis},
   volume = {73},
   year = {2007}
}

TY  - JOUR
ID  - 750291
AU  - Březinová, Lenka - Vlašínová, Helena - Havel, Ladislav - Bohatcová, Irena - Chvátalová, Kateřina - Slanina, Jiří
PY  - 2007
TI  - Formation of lignans in plant cell culture of Schisandra chinensis
JF  - Planta Medica
VL  - 73
IS  - 9
SP  - 1015-1016
EP  - 1015-1016
PB  - Thieme Medical Publishers
SN  - 00320943
KW  - Schisandra
KW  - lignans
KW  - HPLC
N2  - The fruit of Schisandra chinensis, a woody liana, has been used for centuries in traditional Chinese medicine. The fruit is prescribed for the treatment of hepatitis in China. The active principles are lignans with unusual structures derived from dibenzo[a,c]cyclooctadiene. These lignans have been shown to possess a broad range of biological activities. Recently, it has been found that lignan gamma-schizandin strongly inhibits P-glycoprotein, the overexpression of which is the most frequent cause for cancer multidrug resistance. S. chinensis cultures derived from immature zygotic embryos were developed on Murashige and Skoog and VW5 medium. Embryogenic cultures were established on the medium containing thidiazuron, 2,4-dichlorophenoxyacetic acid and benzylaminopurine. An analytical method based on HPLC-UV was used for the determination of six lignans in plant cell cultures. Our previous results showed that the concentration of lignans in plant cell cultures was mostly lower than that in fruit and leaves. We also detected the lignans in liquid culture media, but the amount of lignans inside the cells was substantially higher. In order to release the lignans stored within the cells, we explored the cultivation of five cell lines with neutral polymeric resin, Amberlite XAD2. We found that the addition of Amberlite XAD2 not only notably enlarged portions of extracellular lignans, but also greatly enhanced the production of lignan deoxyschizandrin, whereas the formation of other lignans was increased only moderately (less than 5fold). Importantly, Amberlite XAD-2 did not reduce the growth of cell cultures. Deoxyschizandrin adsorbed on the polymeric resin can be easily recovered and purified without destruction of the cells.
ER  -

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Ladislav HAVEL, Irena BOHATCOVÁ, Kateřina CHVÁTALOVÁ a Jiří SLANINA. Formation of lignans in plant cell culture of Schisandra chinensis. \textit{Planta Medica}. Stuttgart - New York: Thieme Medical Publishers, 2007, roč.~73, č.~9, s.~1015-1016. ISSN~0032-0943.

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