| STROUHAL, Michal, David ŠMAJS, Petra MATĚJKOVÁ, Erica SODERGREN, Anita G. AMIN, Jerrilyn K. HOWELL, Steven J. NORRIS and George M. WEINSTOCK. Genome Differences between Treponema pallidum subsp. pallidum Strain Nichols and T. paraluiscuniculi Strain Cuniculi A. INFECTION AND IMMUNITY. The American Society for Microbiology, 2007, vol. 75, No 12, p. 5859-5866. ISSN 0019-9567. |
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@article{750874,
author = {Strouhal, Michal and Šmajs, David and Matějková, Petra and Sodergren, Erica and Amin, Anita G. and Howell, Jerrilyn K. and Norris, Steven J. and Weinstock, George M.},
article_number = {12},
keywords = {Treponema pallidum; Treponema paraluiscuniculi; restriction analysis},
language = {eng},
issn = {0019-9567},
journal = {INFECTION AND IMMUNITY},
title = {Genome Differences between Treponema pallidum subsp. pallidum Strain Nichols and T. paraluiscuniculi Strain Cuniculi A},
url = {http://iai.asm.org/cgi/content/abstract/75/12/5859},
volume = {75},
year = {2007}
}
TY - JOUR
ID - 750874
AU - Strouhal, Michal - Šmajs, David - Matějková, Petra - Sodergren, Erica - Amin, Anita G. - Howell, Jerrilyn K. - Norris, Steven J. - Weinstock, George M.
PY - 2007
TI - Genome Differences between Treponema pallidum subsp. pallidum Strain Nichols and T. paraluiscuniculi Strain Cuniculi A
JF - INFECTION AND IMMUNITY
VL - 75
IS - 12
SP - 5859-5866
EP - 5859-5866
PB - The American Society for Microbiology
SN - 00199567
KW - Treponema pallidum
KW - Treponema paraluiscuniculi
KW - restriction analysis
UR - http://iai.asm.org/cgi/content/abstract/75/12/5859
N2 - The genome of Treponema paraluiscuniculi strain Cuniculi A was compared to the genome of the syphilis spirochete Treponema pallidum subsp. pallidum strain Nichols using DNA microarray hybridization, whole-genome fingerprinting, and DNA sequencing. Using these approaches, deletions, insertions, and prominent sequence changes were found in 38 gene homologs and six intergenic regions of the Cuniculi A genome when it was compared to the genome of T. pallidum subsp. pallidum Nichols. Most of the observed differences were localized in tpr loci and the vicinity of these loci. In addition, 14 other genes were found to contain frameshift mutations resulting in major changes in protein sequences. Analysis of restriction target sites representing 0.34% of the total genome length and DNA sequencing of three PCR products (0.46% of the total genome length) amplified from Cuniculi A chromosomal regions and comparison to the Nichols genome revealed a sequence similarity of 98.6 to 99.3%. These results are consistent with a close genetic relationship among the T. pallidum strains and subspecies and a strong, but relatively divergent connection between the human and rabbit pathogens.
ER -
STROUHAL, Michal, David ŠMAJS, Petra MATĚJKOVÁ, Erica SODERGREN, Anita G. AMIN, Jerrilyn K. HOWELL, Steven J. NORRIS and George M. WEINSTOCK. Genome Differences between Treponema pallidum subsp. pallidum Strain Nichols and T. paraluiscuniculi Strain Cuniculi A. \textit{INFECTION AND IMMUNITY}. The American Society for Microbiology, 2007, vol.~75, No~12, p.~5859-5866. ISSN~0019-9567.
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