Detection and long-term in vivo monitoring of individual
tumor-specific T cell clones in patients with metastatic
melanoma.

J 2007

Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.

MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK, Roman HÁJEK et. al.

Základní údaje

Originální název

Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.

Název česky

Detekce a dlouhodobé in vivo monitorování individuálních nádorově specifických klonů T lymfocytů u pacientů s metastázujícím melanomem.

Autoři

MICHÁLEK, Jaroslav (203 Česká republika, garant), Ivo KOCÁK (203 Česká republika), Vuk FAIT (203 Česká republika), Jan ŽALOUDÍK (203 Česká republika) a Roman HÁJEK (203 Česká republika)

Vydání

Journal of immunology, Bethesda, American association of immunologists, 2007, 0022-1767

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.068

Kód RIV

RIV/00216224:14110/07:00033038

Organizační jednotka

Lékařská fakulta

UT WoS

000246896300018

Klíčová slova anglicky

metastatic melanoma; T cell

Štítky

metastatic melanoma, T cell

Příznaky

Mezinárodní význam

Změněno: 22. 6. 2009 11:15, Ing. Gabriela Petrovičová

Anotace

ORIG CZ

V originále

We investigated the presence of individual melanoma-specific T cell clones in patients with metastatic melanoma. Ten patients were examined for the presence of melanoma-reactive T cells using dendritic cells loaded with autologous tumor cells. Their specificity was tested using nonradioactive cytotoxicity test. Individual immumodominant T cell clones were identified by the clonotypic assay that combines in vitro cell culture, immunomagnetic sorting of activated IFN-gamma(+) T cells, TCR beta locus-anchored RT-PCR, and clonotypic quantitative PCR. All patients had detectable melanoma-reactive T cells in vitro. Expanded melanoma-reactive T cells demonstrated specific cytotoxic effect against autologous tumor cells in vitro. Three patients experienced objective responses, and their clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8(+) T cell clones with frequencies of 10(-6) to 10(-3) of all circulating CD8+ T cells. Five patients with progressive disease experienced no or temporary presence of circulating melanoma-reactive T cell clones. Thus, circulating immunodominant CD8(+) T cell clones closely correlate with clinical outcome inpatients with metastatic melanoma.

Česky

Detekce a dlouhodobé in vivo monitorování individuálních nádorově specifických klonů T lymfocytů u pacientů s metastázujícím melanomem.

Citovat

MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK a Roman HÁJEK. Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma. Journal of immunology. Bethesda: American association of immunologists, 2007, roč. 2007, č. 1, s. 178-189, 11 s. ISSN 0022-1767.

@article{752159,
   author = {Michálek, Jaroslav and Kocák, Ivo and Fait, Vuk and Žaloudík, Jan and Hájek, Roman},
   article_location = {Bethesda},
   article_number = {1},
   keywords = {metastatic melanoma; T cell},
   language = {eng},
   issn = {0022-1767},
   journal = {Journal of immunology},
   title = {Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/17513726?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum},
   volume = {2007},
   year = {2007}
}

TY  - JOUR
ID  - 752159
AU  - Michálek, Jaroslav - Kocák, Ivo - Fait, Vuk - Žaloudík, Jan - Hájek, Roman
PY  - 2007
TI  - Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.
JF  - Journal of immunology
VL  - 2007
IS  - 1
SP  - 178-189
EP  - 178-189
PB  - American association of immunologists
SN  - 00221767
KW  - metastatic melanoma
KW  - T cell
UR  - http://www.ncbi.nlm.nih.gov/pubmed/17513726?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
N2  - We investigated the presence of individual melanoma-specific T cell clones in patients with metastatic melanoma. Ten patients were examined for the presence of melanoma-reactive T cells using dendritic cells loaded with autologous tumor cells. Their specificity was tested using nonradioactive cytotoxicity test. Individual immumodominant T cell clones were identified by the clonotypic assay that combines in vitro cell culture, immunomagnetic sorting of activated IFN-gamma(+) T cells, TCR beta locus-anchored RT-PCR, and clonotypic quantitative PCR. All patients had detectable melanoma-reactive T cells in vitro. Expanded melanoma-reactive T cells demonstrated specific cytotoxic effect against autologous tumor cells in vitro. Three patients experienced objective responses, and their clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8(+) T cell clones with frequencies of 10(-6) to 10(-3) of all circulating CD8+ T cells. Five patients with progressive disease experienced no or temporary presence of circulating melanoma-reactive T cell clones. Thus, circulating immunodominant CD8(+) T cell clones closely correlate with clinical outcome inpatients with metastatic melanoma.
ER  -

MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK a Roman HÁJEK. Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2007, roč.~2007, č.~1, s.~178-189, 11 s. ISSN~0022-1767.

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