2007
AhR-zprostředkovaná aktivita huminových látek a jejich fotodegradačních produktů
BITTNER, Michal, Klára HILSCHEROVÁ a John, P. GIESYZákladní údaje
Originální název
AhR-zprostředkovaná aktivita huminových látek a jejich fotodegradačních produktů
Název anglicky
AhR-mediated activity of humic substances and their photo-degradation products
Autoři
BITTNER, Michal, Klára HILSCHEROVÁ a John, P. GIESY
Vydání
Toxicita a biodegradabilita odpadů a látek významných ve vodním prostředí - XIII. Toxikologická konference, 2007
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
10511 Environmental sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Přírodovědecká fakulta
Změněno: 16. 7. 2008 13:48, Mgr. Petr Bureš
V originále
Humic substances (HS) are ubiquitous in the environment - they constitute the major components of dissolved organic matter in waters and the major part of materials that comprise soil organic matter. However, some studies indicate that HS can induce a direct adverse effect on human health and hormone-like effects on fish, amphibians and invertebrates. HS molecules contain number of aromatic rings and conjugated double bonds - the so called chromophores. That implies irradiation of dissolved HS can lead to a series of photochemical reactions which can act on the HS itself, or on other substances present in aquatic environment along with HS such as e.g. xenobiotics. In our previous study, we have found significant interactions of pure humic acids (HA) with cytosolic aryl hydrocarbon receptor (AhR). The reporter gene in vitro bioassay with H4IIE-luc cells was used for determination of AhR-mediated activity of HA. H4IIE-luc are stably transfected hepatoma cells with a luciferase reporter gene that is activated via AhR. Irradiation of five different aqueous HA samples were carried out in pyrex tubes using low-pressure mercury lamp. Concentrations of irradiated HA samples were 50 mg/L, and irradiation times were from 0 to 52 hours. In this contribution, we summarize our new findings on alteration of AhR-mediated activity of irradiated HS under the lab conditions. Additionally, supposed mechanisms of observed irradiation-induced decrease in AhR-mediated activity of dissolved HS are discussed.
Anglicky
Humic substances (HS) are ubiquitous in the environment - they constitute the major components of dissolved organic matter in waters and the major part of materials that comprise soil organic matter. However, some studies indicate that HS can induce a direct adverse effect on human health and hormone-like effects on fish, amphibians and invertebrates. HS molecules contain number of aromatic rings and conjugated double bonds - the so called chromophores. That implies irradiation of dissolved HS can lead to a series of photochemical reactions which can act on the HS itself, or on other substances present in aquatic environment along with HS such as e.g. xenobiotics. In our previous study, we have found significant interactions of pure humic acids (HA) with cytosolic aryl hydrocarbon receptor (AhR). The reporter gene in vitro bioassay with H4IIE-luc cells was used for determination of AhR-mediated activity of HA. H4IIE-luc are stably transfected hepatoma cells with a luciferase reporter gene that is activated via AhR. Irradiation of five different aqueous HA samples were carried out in pyrex tubes using low-pressure mercury lamp. Concentrations of irradiated HA samples were 50 mg/L, and irradiation times were from 0 to 52 hours. In this contribution, we summarize our new findings on alteration of AhR-mediated activity of irradiated HS under the lab conditions. Additionally, supposed mechanisms of observed irradiation-induced decrease in AhR-mediated activity of dissolved HS are discussed.
Návaznosti
| GP525/05/P160, projekt VaV |
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| MSM0021622412, záměr |
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