J 2007

The study of properties of HPLC determination of polycyclic aromatic nitrogen heterocycles

ŠVÁBENSKÝ, Radim, Kamila KOČÍ a Zdeněk ŠIMEK

Základní údaje

Originální název

The study of properties of HPLC determination of polycyclic aromatic nitrogen heterocycles

Název česky

Studium podmínek HPLC stanovení polycyclických aromatických dusíkatých heterocyklů

Autoři

ŠVÁBENSKÝ, Radim (203 Česká republika), Kamila KOČÍ (203 Česká republika) a Zdeněk ŠIMEK (203 Česká republika, garant)

Vydání

International Journal of Environmental Analytical Chemistry, 2007, 1029-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.026

Kód RIV

RIV/00216224:14310/07:00023783

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000244920500003

Klíčová slova anglicky

polycyclic aromatic nitrogen heterocycles; azaarenes; PANHs; PAHs;

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 31. 1. 2008 15:55, doc. RNDr. Zdeněk Šimek, CSc.

Anotace

V originále

The study of the separation of polycyclic aromatic nitrogen heterocycles (PANHs) by reversephase liquid chromatography with an octadecyl stationary phase is presented. The retention behaviour of a mixture of PANHs was studied under different chromatographic conditions. A mixture of phosphate buffer/acetonitrile was used as mobile phase in isocratic and gradient modes. The effect of different pH mobile phase in the range from 2.5 to 6.5 has been investigated to describe retention changes of PANHs as a function of their acid/base properties. Different concentrations of phosphate buffer as a component of the mobile phase were used to study the effect of ionic strength. Very good RP-HPLC separation of 24 PANHs and 16 EPA polycyclic aromatic hydrocarbons (PAHs) was obtained without a pre-separation step in a test mixture and the extract of a real soil sample. Limits of detection of PANHs obtained by two detection techniques, ultraviolet-diode array detection (UV-DAD) and fluorescence detector (FD), are compared. The proposed method is tested with a real soil sample.

Česky

The study of the separation of polycyclic aromatic nitrogen heterocycles (PANHs) by reversephase liquid chromatography with an octadecyl stationary phase is presented. The retention behaviour of a mixture of PANHs was studied under different chromatographic conditions. A mixture of phosphate buffer/acetonitrile was used as mobile phase in isocratic and gradient modes. The effect of different pH mobile phase in the range from 2.5 to 6.5 has been investigated to describe retention changes of PANHs as a function of their acid/base properties. Different concentrations of phosphate buffer as a component of the mobile phase were used to study the effect of ionic strength. Very good RP-HPLC separation of 24 PANHs and 16 EPA polycyclic aromatic hydrocarbons (PAHs) was obtained without a pre-separation step in a test mixture and the extract of a real soil sample. Limits of detection of PANHs obtained by two detection techniques, ultraviolet-diode array detection (UV-DAD) and fluorescence detector (FD), are compared. The proposed method is tested with a real soil sample.

Návaznosti

MSM0021622412, záměr
Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni