2007
The study of properties of HPLC determination of polycyclic aromatic nitrogen heterocycles
ŠVÁBENSKÝ, Radim, Kamila KOČÍ a Zdeněk ŠIMEKZákladní údaje
Originální název
The study of properties of HPLC determination of polycyclic aromatic nitrogen heterocycles
Název česky
Studium podmínek HPLC stanovení polycyclických aromatických dusíkatých heterocyklů
Autoři
ŠVÁBENSKÝ, Radim (203 Česká republika), Kamila KOČÍ (203 Česká republika) a Zdeněk ŠIMEK (203 Česká republika, garant)
Vydání
International Journal of Environmental Analytical Chemistry, 2007, 1029-0397
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.026
Kód RIV
RIV/00216224:14310/07:00023783
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000244920500003
Klíčová slova anglicky
polycyclic aromatic nitrogen heterocycles; azaarenes; PANHs; PAHs;
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 31. 1. 2008 15:55, doc. RNDr. Zdeněk Šimek, CSc.
V originále
The study of the separation of polycyclic aromatic nitrogen heterocycles (PANHs) by reversephase liquid chromatography with an octadecyl stationary phase is presented. The retention behaviour of a mixture of PANHs was studied under different chromatographic conditions. A mixture of phosphate buffer/acetonitrile was used as mobile phase in isocratic and gradient modes. The effect of different pH mobile phase in the range from 2.5 to 6.5 has been investigated to describe retention changes of PANHs as a function of their acid/base properties. Different concentrations of phosphate buffer as a component of the mobile phase were used to study the effect of ionic strength. Very good RP-HPLC separation of 24 PANHs and 16 EPA polycyclic aromatic hydrocarbons (PAHs) was obtained without a pre-separation step in a test mixture and the extract of a real soil sample. Limits of detection of PANHs obtained by two detection techniques, ultraviolet-diode array detection (UV-DAD) and fluorescence detector (FD), are compared. The proposed method is tested with a real soil sample.
Česky
The study of the separation of polycyclic aromatic nitrogen heterocycles (PANHs) by reversephase liquid chromatography with an octadecyl stationary phase is presented. The retention behaviour of a mixture of PANHs was studied under different chromatographic conditions. A mixture of phosphate buffer/acetonitrile was used as mobile phase in isocratic and gradient modes. The effect of different pH mobile phase in the range from 2.5 to 6.5 has been investigated to describe retention changes of PANHs as a function of their acid/base properties. Different concentrations of phosphate buffer as a component of the mobile phase were used to study the effect of ionic strength. Very good RP-HPLC separation of 24 PANHs and 16 EPA polycyclic aromatic hydrocarbons (PAHs) was obtained without a pre-separation step in a test mixture and the extract of a real soil sample. Limits of detection of PANHs obtained by two detection techniques, ultraviolet-diode array detection (UV-DAD) and fluorescence detector (FD), are compared. The proposed method is tested with a real soil sample.
Návaznosti
MSM0021622412, záměr |
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