2008
Genetic variations and plasma levels of the Gelatinase A (matrix metalloproteinase-2) and Gelatinase B (matrix metalloproteinase-9) in proliferative diabetic retinopathy
BERÁNEK, Michal, Petr KOLÁŘ, Svatava TSCHÖPLOVÁ, Kateřina KAŇKOVÁ, Anna VAŠKŮ et. al.Základní údaje
Originální název
Genetic variations and plasma levels of the Gelatinase A (matrix metalloproteinase-2) and Gelatinase B (matrix metalloproteinase-9) in proliferative diabetic retinopathy
Název česky
Genetic variations and plasma levels of the Gelatinase A (matrix metalloproteinase-2) and Gelatinase B (matrix metalloproteinase-9) in proliferative diabetic retinopathy
Autoři
BERÁNEK, Michal (203 Česká republika), Petr KOLÁŘ (203 Česká republika, domácí), Svatava TSCHÖPLOVÁ (203 Česká republika, domácí), Kateřina KAŇKOVÁ (203 Česká republika, garant, domácí) a Anna VAŠKŮ (203 Česká republika, domácí)
Vydání
Molecular Vision, 2008, 1090-0535
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30202 Endocrinology and metabolism
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.464
Kód RIV
RIV/00216224:14110/08:00024690
Organizační jednotka
Lékařská fakulta
UT WoS
000257890500002
Klíčová slova anglicky
retinopathy; MMP; diabetes
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 29. 10. 2012 10:22, prof. MUDr. Petr Kolář, Ph.D.
V originále
Purpose: The matrix metalloproteinases (MMPs) are postulated to be involved in the development of retinal angiogenesis through the regulation of extracellular matrix. The objective of the present study was to test for a possible association of five single nucleotide polymorphisms (SNPs) in the MMP-2 gene and two polymorphisms in the MMP-9 gene with proliferative diabetic retinopathy (PDR) and to determine their plasma levels. Methods: Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies determined in a study comprising three groups of Caucasian subjects (total n=490, diabetics with and without PDR and non-diabetics). The plasma levels of the MMP-2 and -9 proteins were analyzed by ELISA. Results: Neither MMP-2 nor MMP-9 SNPs revealed significant association with PDR in single-locus comparisons; similarly, MMP-2 haplotype frequencies did not differed significantly between groups. Both MMP-2 and MMP-9 plasma levels showed statistically significant differences among the studied groups (p<0.001 and p=0.001, respectively) with highest levels in the PDR group. MMP-2 plasma levels were significantly higher in carriers of either the -1306CC and CT genotypes (p=0.009) or CGCG haplotype (p=0.043). Conclusions: These findings indicate that genotype- and haplotype-specific effects on the MMP-2 gene expression corresponding with its plasma levels may contribute to the susceptibility to PDR.
Česky
Purpose: The matrix metalloproteinases (MMPs) are postulated to be involved in the development of retinal angiogenesis through the regulation of extracellular matrix. The objective of the present study was to test for a possible association of five single nucleotide polymorphisms (SNPs) in the MMP-2 gene and two polymorphisms in the MMP-9 gene with proliferative diabetic retinopathy (PDR) and to determine their plasma levels. Methods: Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies determined in a study comprising three groups of Caucasian subjects (total n=490, diabetics with and without PDR and non-diabetics). The plasma levels of the MMP-2 and -9 proteins were analyzed by ELISA. Results: Neither MMP-2 nor MMP-9 SNPs revealed significant association with PDR in single-locus comparisons; similarly, MMP-2 haplotype frequencies did not differed significantly between groups. Both MMP-2 and MMP-9 plasma levels showed statistically significant differences among the studied groups (p<0.001 and p=0.001, respectively) with highest levels in the PDR group. MMP-2 plasma levels were significantly higher in carriers of either the -1306CC and CT genotypes (p=0.009) or CGCG haplotype (p=0.043). Conclusions: These findings indicate that genotype- and haplotype-specific effects on the MMP-2 gene expression corresponding with its plasma levels may contribute to the susceptibility to PDR.
Návaznosti
| GP303/05/P523, projekt VaV |
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