2008
Multidetection platform for microcolumn separation of proteins and peptides
PREISLER, JanZákladní údaje
Originální název
Multidetection platform for microcolumn separation of proteins and peptides
Název česky
Multidetection platform for microcolumn separation of proteins and peptides
Autoři
PREISLER, Jan (203 Česká republika, garant, domácí)
Vydání
2008
Další údaje
Jazyk
angličtina
Typ výsledku
Vyžádané přednášky
Obor
10406 Analytical chemistry
Stát vydavatele
Irsko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/08:00033542
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
CE; ICP; MALDI MS; interface; off-line coupling
Štítky
Změněno: 11. 4. 2013 09:44, prof. Mgr. Jan Preisler, Ph.D.
V originále
This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. The developed instrumentation and methods will be tested on analysis of metal complexes, protein complexes with metals and phosphorylated proteins.
Česky
This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. The developed instrumentation and methods will be tested on analysis of metal complexes, protein complexes with metals and phosphorylated proteins.
Návaznosti
| LC06035, projekt VaV |
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| MSM0021622411, záměr |
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| MSM0021622412, záměr |
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| MSM0021622415, záměr |
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