Quantification of t-tubule area and protein distribution in rat
cardiac ventricular myocytes

J 2008

Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes

PÁSEK, Michal, F. BRETTE, A. NELSON, C. PEARCE, A. QAISER et. al.

Základní údaje

Originální název

Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes

Název česky

Stanovení plochy t-tubulů a distribuce transportních bílkovin u srdečních komorových buněk potkana

Autoři

PÁSEK, Michal (203 Česká republika, garant, domácí), F. BRETTE (250 Francie), A. NELSON (826 Velká Británie a Severní Irsko), C. PEARCE (826 Velká Británie a Severní Irsko), A. QAISER (826 Velká Británie a Severní Irsko), G. CHRISTÉ (250 Francie) a C. ORCHARD (826 Velká Británie a Severní Irsko)

Vydání

Progress in Biophysics and Molecular Biology, Great Britain, Elsevier Ltd. 2008, 0079-6107

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.388

Kód RIV

RIV/00216224:14110/08:00024218

Organizační jednotka

Lékařská fakulta

UT WoS

000254598000014

Klíčová slova anglicky

cardiac cell; transverse-axial tubular system; tubular membrane area; tubular membrane capacitance

Štítky

Cardiac cell, transverse-axial tubular system, tubular membrane area, tubular membrane capacitance

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 12. 2012 17:27, doc. Ing. Michal Pásek, Ph.D.

Anotace

ORIG CZ

V originále

The transverse (t-) tubules of cardiac ventricular myocytes are invaginations of the surface membrane that form a complex network within the cell. Many of the key proteins involved in excitation-contraction coupling appear to be located predominantly at the t-tubule membrane. Despite their importance, the fraction of cell membrane within the ttubules remains unclear: measurement of cell capacitance following detubulation suggests 32%, whereas optical measurements suggest up to 65%. We have, therefore, investigated the factors that may account for this discrepancy. Calculation of the combinations of t-tubule radius, length and density that produce t-tubular membrane fractions of 32% or 56% suggest that the true fraction is at the upper end of this range. Assessment of detubulation using confocal and electron microscopy suggests that incomplete detubulation can account for some, but not all of the difference.

Česky

Práce prezentuje využití matematického modelu pro určení frakční plochy tubulární membrány a pro odhad distribuce transportních bílkovin mezi periferní a tubulární membránou u srdečních komorových buněk laboratorního potkana.

Návaznosti

MSM0021622402, záměr

Název: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časná diagnostika a léčba kardiovaskulárních chorob

Citovat

PÁSEK, Michal, F. BRETTE, A. NELSON, C. PEARCE, A. QAISER, G. CHRISTÉ a C. ORCHARD. Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes. Progress in Biophysics and Molecular Biology. Great Britain: Elsevier Ltd., 2008, roč. 96, 1-3, s. 244-257. ISSN 0079-6107.

@article{770902,
   author = {Pásek, Michal and Brette, F. and Nelson, A. and Pearce, C. and Qaiser, A. and Christé, G. and Orchard, C.},
   article_location = {Great Britain},
   article_number = {1-3},
   keywords = {cardiac cell; transverse-axial tubular system; tubular membrane area; tubular membrane capacitance},
   language = {eng},
   issn = {0079-6107},
   journal = {Progress in Biophysics and Molecular Biology},
   title = {Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes},
   volume = {96},
   year = {2008}
}

TY  - JOUR
ID  - 770902
AU  - Pásek, Michal - Brette, F. - Nelson, A. - Pearce, C. - Qaiser, A. - Christé, G. - Orchard, C.
PY  - 2008
TI  - Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes
JF  - Progress in Biophysics and Molecular Biology
VL  - 96
IS  - 1-3
SP  - 244-257
EP  - 244-257
PB  - Elsevier Ltd.
SN  - 00796107
KW  - cardiac cell
KW  - transverse-axial tubular system
KW  - tubular membrane area
KW  - tubular membrane capacitance
N2  - The transverse (t-) tubules of cardiac ventricular myocytes are invaginations of the surface membrane that form a complex network within the cell. Many of the key proteins involved in excitation-contraction coupling appear to be located predominantly at the t-tubule membrane. Despite their importance, the fraction of cell membrane within the ttubules remains unclear: measurement of cell capacitance following detubulation suggests 32%, whereas optical measurements suggest up to 65%. We have, therefore, investigated the factors that may account for this discrepancy. Calculation of the combinations of t-tubule radius, length and density that produce t-tubular membrane fractions of 32% or 56% suggest that the true fraction is at the upper end of this range. Assessment of detubulation using confocal and electron microscopy suggests that incomplete detubulation can account for some, but not all of the difference.
ER  -

PÁSEK, Michal, F. BRETTE, A. NELSON, C. PEARCE, A. QAISER, G. CHRISTÉ a C. ORCHARD. Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes. \textit{Progress in Biophysics and Molecular Biology}. Great Britain: Elsevier Ltd., 2008, roč.~96, 1-3, s.~244-257. ISSN~0079-6107.

Podrobný výpis o publikaci