BÜYÜKGÜZEL, Ender, Pavel HYRŠL and Kemal BÜYÜKGÜZEL. The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae. In SEB Book of abstracts. 2008.
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Basic information
Original name The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae
Name in Czech Vliv inhibitorů biosyntézy eicosanoidů na oxidativní stres, antioxidační odpověď a proteinové spektrum hemolymfy larev Galleria mellonella
Authors BÜYÜKGÜZEL, Ender (792 Turkey), Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution) and Kemal BÜYÜKGÜZEL (792 Turkey).
Edition SEB Book of abstracts, 2008.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30105 Physiology
Country of publisher France
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/08:00042007
Organization unit Faculty of Science
Keywords in English Galleria mellonella; esculetin; malondialdehyde; glutathione S-transferase; hemolymph proteins
Tags esculetin, Galleria mellonella, glutathione S-transferase, hemolymph proteins, malondialdehyde
Tags International impact
Changed by Changed by: doc. RNDr. Pavel Hyršl, Ph.D., učo 9982. Changed: 28/4/2011 16:16.
Abstract
Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in the presence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations caused significant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae. The quantity of the 45 kDa protein fraction from total 16 protein detected was increased with increasing esculetin concentrations. An increase after phenidone and dexamethasone exposure was detected only at 0.001 and 0.01% concentration. Other main hemolymph proteins such as ApoLP-I and storage proteins were not altered in larvae reared on diets amended with the EBIs. We suggest that 45 kDa protein may be an oxidative stress-related protein in hemolymph of G. mellonella. This is the first demonstration that antioxidative responses to a EBI exposure are modulated by a physiological system that includes eicosanoid biosynthesis.
Abstract (in Czech)
Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in the presence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations caused significant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae. The quantity of the 45 kDa protein fraction from total 16 protein detected was increased with increasing esculetin concentrations. An increase after phenidone and dexamethasone exposure was detected only at 0.001 and 0.01% concentration. Other main hemolymph proteins such as ApoLP-I and storage proteins were not altered in larvae reared on diets amended with the EBIs. We suggest that 45 kDa protein may be an oxidative stress-related protein in hemolymph of G. mellonella. This is the first demonstration that antioxidative responses to a EBI exposure are modulated by a physiological system that includes eicosanoid biosynthesis.
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