2008
Perphenazine-Induced block of sodium and transient outward potassium current: experimental and simulation study
BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Michal PÁSEK, Dagmar JANSOVÁ, Milena ŠIMURDOVÁ et. al.Základní údaje
Originální název
Perphenazine-Induced block of sodium and transient outward potassium current: experimental and simulation study
Název česky
Perfenazinem způsobená blokáda sodíkového proudu a přechodného proudu draslíku z buňky: experimentální a simulační studie
Autoři
BÉBAROVÁ, Markéta (203 Česká republika, garant, domácí), Peter MATEJOVIČ (703 Slovensko, domácí), Michal PÁSEK (203 Česká republika), Dagmar JANSOVÁ (203 Česká republika, domácí), Milena ŠIMURDOVÁ (203 Česká republika, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí) a Jiří ŠIMURDA (203 Česká republika, domácí)
Vydání
Analysis of Biomedical Signals and Images, Brno, Vutium Press, 2008, 1211-412X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30105 Physiology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/08:00024258
Organizační jednotka
Lékařská fakulta
UT WoS
000303717200018
Klíčová slova anglicky
sodium current;potassium outward current;simulation
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 19. 1. 2013 22:00, doc. MUDr. Markéta Bébarová, Ph.D.
V originále
The effect of a neuroleptic drug perphenazine on sodium current INa and transient outward potassium current Ito was studied in rat ventricular myocytes at room temperature. Perphenazine reversibly blocked INa (reducing its amplitude; IC50 = 1.24+/-0.10 micromol/l, nH = 0.99+/-0.08) and Ito (accelerating its apparent inactivation with a slight decrease of its amplitude; IC50 = 38.2+/-3.5 micromol/l, nH = 0.75+/-0.07, evaluated from the changes of time integral). Both INa- and Ito-block was use- and frequency-dependent (20%-increase of INablock and 10%-increase of Ito-block under 0.3 and 10 micromol/l perphenazine, respectively, at the stimulation frequency of 3.3 Hz applied after a 15-s rest). The results of quantitative modelling suggest that perphenazine interacts with INa-channels in inactivated states, and with Ito-channels in both open and open-inactivated states.
Česky
The effect of a neuroleptic drug perphenazine on sodium current INa and transient outward potassium current Ito was studied in rat ventricular myocytes at room temperature. Perphenazine reversibly blocked INa (reducing its amplitude; IC50 = 1.24+/-0.10 micromol/l, nH = 0.99+/-0.08) and Ito (accelerating its apparent inactivation with a slight decrease of its amplitude; IC50 = 38.2+/-3.5 micromol/l, nH = 0.75+/-0.07, evaluated from the changes of time integral). Both INa- and Ito-block was use- and frequency-dependent (20%-increase of INablock and 10%-increase of Ito-block under 0.3 and 10 micromol/l perphenazine, respectively, at the stimulation frequency of 3.3 Hz applied after a 15-s rest). The results of quantitative modelling suggest that perphenazine interacts with INa-channels in inactivated states, and with Ito-channels in both open and open-inactivated states.
Návaznosti
| GA305/04/1385, projekt VaV |
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| MSM0021622402, záměr |
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