Genotype variability of the enterotoxin H-positive
Staphylococcus aureus strains isolated from patients and foods
in the Czech Republic

D 2008

Genotype variability of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic

RŮŽIČKOVÁ, Vladislava; Renata KARPÍŠKOVÁ; Pavla HOLOCHOVÁ; Markéta POSPÍŠILOVÁ; Jiří DOŠKAŘ et. al.

Základní údaje

Originální název

Genotype variability of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic

Název česky

Genotypová variabilita enterotoxin H-pozitivních kmenů Staphylococcus aureus izolovaných z pacientů a potravin v České republice

Autoři

RŮŽIČKOVÁ, Vladislava (203 Česká republika, garant); Renata KARPÍŠKOVÁ (203 Česká republika); Pavla HOLOCHOVÁ (203 Česká republika); Markéta POSPÍŠILOVÁ (203 Česká republika); Jiří DOŠKAŘ (203 Česká republika) a Roman PANTŮČEK (203 Česká republika)

Vydání

Cairns, Australia, 13th International Symposium on Staphylococci and Staphylococcal Infections, s. 41-41, 2008

Nakladatel

Australian Society for Antimicrobials

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/08:00026427

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus aureus; enterotoxin H

Štítky

enterotoxin H, Staphylococcus aureus

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

Objective: Staphylococcal enterotoxin H (SEH) is a member of the extracellular toxin family secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Genetic properties of the 32 SEH-positive S. aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were studied. One strain, detected in feta cheese, was implicated in a case of SFP. Methods: Genotyping of strains was performed by PCR detection of the genes for the enterotoxins SEA-SEJ, spa gene polymorphism analysis, PFGE profiling, ERIC2-PCR fingerprinting and prophage carriage. Results: Sixteen of 28 strains carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec genes and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of various genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one isolate from raw meat and two strains from pizza. Conclusions: Our results show that the SEH-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.

Česky

neuvedeno

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

RŮŽIČKOVÁ, Vladislava; Renata KARPÍŠKOVÁ; Pavla HOLOCHOVÁ; Markéta POSPÍŠILOVÁ; Jiří DOŠKAŘ a Roman PANTŮČEK. Genotype variability of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic. In 13th International Symposium on Staphylococci and Staphylococcal Infections. Cairns, Australia: Australian Society for Antimicrobials, 2008, s. 41.

@inproceedings{778555,
   author = {Růžičková, Vladislava and Karpíšková, Renata and Holochová, Pavla and Pospíšilová, Markéta and Doškař, Jiří and Pantůček, Roman},
   address = {Cairns, Australia},
   booktitle = {13th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Staphylococcus aureus; enterotoxin H},
   language = {eng},
   location = {Cairns, Australia},
   pages = {41-41},
   publisher = {Australian Society for Antimicrobials},
   title = {Genotype variability of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic},
   url = {http://www.isssi2008.com/},
   year = {2008}
}

TY  - CONF
ID  - 778555
AU  - Růžičková, Vladislava - Karpíšková, Renata - Holochová, Pavla - Pospíšilová, Markéta - Doškař, Jiří - Pantůček, Roman
PY  - 2008
TI  - Genotype variability of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
PB  - Australian Society for Antimicrobials
CY  - Cairns, Australia
KW  - Staphylococcus aureus
KW  - enterotoxin H
UR  - http://www.isssi2008.com/
N2  - Objective: Staphylococcal enterotoxin H (SEH) is a member of the extracellular toxin family secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Genetic properties of the 32 SEH-positive S. aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were studied. One strain, detected in feta cheese, was implicated in a case of SFP. Methods: Genotyping of strains was performed by PCR detection of the genes for the enterotoxins SEA-SEJ, spa gene polymorphism analysis, PFGE profiling, ERIC2-PCR fingerprinting and prophage carriage. Results: Sixteen of 28 strains carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec genes and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of various genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one isolate from raw meat and two strains from pizza. Conclusions: Our results show that the SEH-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
ER  -

RŮŽIČKOVÁ, Vladislava; Renata KARPÍŠKOVÁ; Pavla HOLOCHOVÁ; Markéta POSPÍŠILOVÁ; Jiří DOŠKAŘ a Roman PANTŮČEK. Genotype variability of the enterotoxin H-positive $<$I$>$Staphylococcus aureus$<$/I$>$ strains isolated from patients and foods in the Czech Republic. In \textit{13th International Symposium on Staphylococci and Staphylococcal Infections}. Cairns, Australia: Australian Society for Antimicrobials, 2008, s.~41.

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