Characteristics of Panton - Valentine leukocidin positive
Staphylococcus aureus strains isolated in the Czech Republic

D 2008

Characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic

PANTŮČEK, Roman, Ivana MACHOVÁ, Jana KAHÁNKOVÁ, Petr PETRÁŠ, Lucie KUNTOVÁ et. al.

Základní údaje

Originální název

Characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic

Název česky

Charakterizace kmenů Staphylococcus aureus pozitivních na Pantonův-Valentinův leukocidin v České republice

Autoři

PANTŮČEK, Roman (203 Česká republika, garant), Ivana MACHOVÁ (203 Česká republika), Jana KAHÁNKOVÁ (203 Česká republika), Petr PETRÁŠ (203 Česká republika), Lucie KUNTOVÁ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

Cairns, Australia, 13th International Symposium on Staphylococci and Staphylococcal Infections, s. 66-66, 2008

Nakladatel

Australian Society for Antimicrobials

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/08:00026429

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus aureus; Panton - Valentine leukocidin; MRSA; community acquired infection

Štítky

community acquired infection, MRSA, Panton - Valentine leukocidin, Staphylococcus aureus

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

Objectives: Phenotypic and genotypic characteristics of PVL positive S. aureus strains isolated from clinical specimens in the Czech Republic between January 2004 and April 2008 are described. Methods: Carriage of the lukS-PV and lukF-PV genes for PVL, and mecA gene was tested by a multiplex PCR assay. The phenotypic and genotypic methods were used for detection of superantigens (enterotoxin A-J, TSST-1, and exfoliatins A and B). The PVL positive MRSA strains were characterized in detail by PFGE, spa typing, SCCmec typing, arcA gene detection, agr typing, plasmid typing, and prophage typing. Results: The presence of the lukS-PV and lukF-PV genes was detected in 159 (6.8 %) of 2337 S. aureus strains. Only 21 strains were MRSA, 17 of them were SCCmec type IV, three SCCmec V and one harbored a composite SCCmec with ccrB and ccrC recombinases. Genotypic properties of PVL positive MRSA strains were correlated with the known European caMRSA types. In addition to the predominant spa-type t008 (38 %) exhibiting the characteristics of USA300 clone and t044 (14 %) widely disseminated in Central Europe, other spa-types were sporadically represented by t002, t003, t186, t311, t433 and t667 among the caMRSA isolates. Conclusions: We found 159 PVL positive MRSA and MSSA isolates, mainly from lesions, wound infection, skin and soft tissue abscesses, and abscessing pneumonia. Four pneumonia cases were fatal, three causative strains were MRSA, with two of them related to clone USA300. Most (79 %) PVL positive strains were phage typeable and were predominantly classified into phage group II (52 %). The lukS-PV and lukF-PV genes were in all analysed strains associated with the Sa2 type prophages of serogroups A or F.

Česky

neuvedeno

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

PANTŮČEK, Roman, Ivana MACHOVÁ, Jana KAHÁNKOVÁ, Petr PETRÁŠ, Lucie KUNTOVÁ a Jiří DOŠKAŘ. Characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic. In 13th International Symposium on Staphylococci and Staphylococcal Infections. Cairns, Australia: Australian Society for Antimicrobials, 2008, s. 66.

@inproceedings{778558,
   author = {Pantůček, Roman and Machová, Ivana and Kahánková, Jana and Petráš, Petr and Kuntová, Lucie and Doškař, Jiří},
   address = {Cairns, Australia},
   booktitle = {13th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Staphylococcus aureus; Panton - Valentine leukocidin; MRSA; community acquired infection},
   language = {eng},
   location = {Cairns, Australia},
   pages = {66-66},
   publisher = {Australian Society for Antimicrobials},
   title = {Characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic},
   url = {http://www.isssi2008.com/},
   year = {2008}
}

TY  - JOUR
ID  - 778558
AU  - Pantůček, Roman - Machová, Ivana - Kahánková, Jana - Petráš, Petr - Kuntová, Lucie - Doškař, Jiří
PY  - 2008
TI  - Characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic
PB  - Australian Society for Antimicrobials
CY  - Cairns, Australia
KW  - Staphylococcus aureus
KW  - Panton - Valentine leukocidin
KW  - MRSA
KW  - community acquired infection
UR  - http://www.isssi2008.com/
N2  - Objectives: Phenotypic and genotypic characteristics of PVL positive S. aureus strains isolated from clinical specimens in the Czech Republic between January 2004 and April 2008 are described. Methods: Carriage of the lukS-PV and lukF-PV genes for PVL, and mecA gene was tested by a multiplex PCR assay. The phenotypic and genotypic methods were used for detection of superantigens (enterotoxin A-J, TSST-1, and exfoliatins A and B). The PVL positive MRSA strains were characterized in detail by PFGE, spa typing, SCCmec typing, arcA gene detection, agr typing, plasmid typing, and prophage typing. Results: The presence of the lukS-PV and lukF-PV genes was detected in 159 (6.8 %) of 2337 S. aureus strains. Only 21 strains were MRSA, 17 of them were SCCmec type IV, three SCCmec V and one harbored a composite SCCmec with ccrB and ccrC recombinases. Genotypic properties of PVL positive MRSA strains were correlated with the known European caMRSA types. In addition to the predominant spa-type t008 (38 %) exhibiting the characteristics of USA300 clone and t044 (14 %) widely disseminated in Central Europe, other spa-types were sporadically represented by t002, t003, t186, t311, t433 and t667 among the caMRSA isolates. Conclusions: We found 159 PVL positive MRSA and MSSA isolates, mainly from lesions, wound infection, skin and soft tissue abscesses, and abscessing pneumonia. Four pneumonia cases were fatal, three causative strains were MRSA, with two of them related to clone USA300. Most (79 %) PVL positive strains were phage typeable and were predominantly classified into phage group II (52 %). The lukS-PV and lukF-PV genes were in all analysed strains associated with the Sa2 type prophages of serogroups A or F.
ER  -

PANTŮČEK, Roman, Ivana MACHOVÁ, Jana KAHÁNKOVÁ, Petr PETRÁŠ, Lucie KUNTOVÁ a Jiří DOŠKAŘ. Characteristics of Panton - Valentine leukocidin positive $<$I$>$Staphylococcus aureus$<$/I$>$ strains isolated in the Czech Republic. In \textit{13th International Symposium on Staphylococci and Staphylococcal Infections}. Cairns, Australia: Australian Society for Antimicrobials, 2008, s.~66.

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