Evaluation of (GTG)5-PCR for rapid identification of
Streptococcus mutans

J 2008

Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

ŠVEC, Pavel, Dana NOVÁKOVÁ, Lenka ŽÁČKOVÁ, Martina KUKLETOVÁ, Ivo SEDLÁČEK et. al.

Basic information

Original name

Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

Name in Czech

(GTG)5-PCR pro rychlou identifikaci druhu Streptococcus mutans

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor), Dana NOVÁKOVÁ (203 Czech Republic), Lenka ŽÁČKOVÁ (203 Czech Republic), Martina KUKLETOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)

Edition

Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Springer, 2008, 0003-6072

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.673

RIV identification code

RIV/00216224:14310/08:00026509

Organization unit

Faculty of Science

UT WoS

000259670400008

Keywords in English

Dental caries; (GTG)5-PCR; Identification; Automated ribotyping; Streptococcus mutans

Abstract

ORIG CZ

V originále

Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.

In Czech

Metoda rep-PCR s primerem (GTG)5 byla použita pro analýzu bakterií izolovaných ze zubního kazu dětí. Tyto kmeny byly dále analyzovány automatickou ribotypizací (RiboPrinter) a analýzou celkových buněčných proteinů. Na základě získaných výsledků se (GTG)5-PCR ukázala jako vhodná pro identifikaci druhu Streptococcus mutans.

Links

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

1M0528, research and development project

Name: Stomatologické výzkumné centrum

Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre

Citovat

ŠVEC, Pavel, Dana NOVÁKOVÁ, Lenka ŽÁČKOVÁ, Martina KUKLETOVÁ and Ivo SEDLÁČEK. Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Springer, 2008, vol. 94, No 4, p. 573-579. ISSN 0003-6072.

@article{781895,
   author = {Švec, Pavel and Nováková, Dana and Žáčková, Lenka and Kukletová, Martina and Sedláček, Ivo},
   article_number = {4},
   keywords = {Dental caries; (GTG)5-PCR; Identification; Automated ribotyping; Streptococcus mutans},
   language = {eng},
   issn = {0003-6072},
   journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
   title = {Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans},
   url = {http://www.springerlink.com/content/268q817531426227/?p=e909337e76ae416fbddd48f749191788&pi=7},
   volume = {94},
   year = {2008}
}

TY  - JOUR
ID  - 781895
AU  - Švec, Pavel - Nováková, Dana - Žáčková, Lenka - Kukletová, Martina - Sedláček, Ivo
PY  - 2008
TI  - Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans
JF  - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL  - 94
IS  - 4
SP  - 573-579
EP  - 573-579
PB  - Springer
SN  - 00036072
KW  - Dental caries
KW  - (GTG)5-PCR
KW  - Identification
KW  - Automated ribotyping
KW  - Streptococcus mutans
UR  - http://www.springerlink.com/content/268q817531426227/?p=e909337e76ae416fbddd48f749191788&pi=7
N2  - Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.
ER  -

ŠVEC, Pavel, Dana NOVÁKOVÁ, Lenka ŽÁČKOVÁ, Martina KUKLETOVÁ and Ivo SEDLÁČEK. Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Springer, 2008, vol.~94, No~4, p.~573-579. ISSN~0003-6072.

Detailed Information on Publication Record