Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer

VAŇHARA, Petr, Eva LINCOVÁ, Karel SOUČEK and Jan ŠMARDA. Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer. In Stem cells, cancer and aging. Singapore: Keystone symposia, International Society of Differentiation, 2008, p. 89-90.

Basic information

• Original name: Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer
• Name in Czech: Regulace osteoklastové diferenciaci proteiny TGFbeta sekretovanymi nadory prostaty
• Authors: VAŇHARA, Petr, Eva LINCOVÁ, Karel SOUČEK and Jan ŠMARDA.
• Edition: Singapore, Stem cells, cancer and aging, p. 89-90, 2008.
• Publisher: Keystone symposia, International Society of Differentiation

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: Singapore
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords in English: TGF; GDF-15; BMP-7; TGFb1; prostate cancer; osteoclast
• Tags: BMP-7, GDF-15, osteoclast, prostate cancer, TGF, TGFb1
• Tags: International impact, Reviewed

Abstract

Prostate caner is a common disease in western countries. Primary prostate tumors preferentially disseminate to bones. Homing and activity of cancer cells in the bone microenvironment as well as specific mechanisms used by tumor cells remain to be elucidated. This study aims to describe the effects of three members of TGFb protein family (TGFb, BMP-7 and GDF-15) on osteoclast differentiation of murine cell line RAW264.7. These proteins are secreted by prostate cancer cell line LNCaP, possess strong morphogenic effects and are supposed to influence bone microenvironment significantly. TGFb, BMP-7 or GDF-15 affected efficiency of osteoclast differentiation strongly. While TGFb treatment stimulated RANKL-induced differentiation, BMP-7 and GDF-15 cytokines clearly reduced differentiation potential of RAW264.7 cells to active osteoclasts. In contrast to RANKL/TGFb that induced expression of several osteoclast-associated genes, RANKL/GDF-15 and RANKL/BMP-7 suppressed it. According to these we document that different members of the TGFb protein family differently regulate signaling in osteoclasts and consequently modulate the final cellular answer.

Abstract (in Czech)

Prostate caner is a common disease in western countries. Primary prostate tumors preferentially disseminate to bones. Homing and activity of cancer cells in the bone microenvironment as well as specific mechanisms used by tumor cells remain to be elucidated. This study aims to describe the effects of three members of TGFb protein family (TGFb, BMP-7 and GDF-15) on osteoclast differentiation of murine cell line RAW264.7. These proteins are secreted by prostate cancer cell line LNCaP, possess strong morphogenic effects and are supposed to influence bone microenvironment significantly. TGFb, BMP-7 or GDF-15 affected efficiency of osteoclast differentiation strongly. While TGFb treatment stimulated RANKL-induced differentiation, BMP-7 and GDF-15 cytokines clearly reduced differentiation potential of RAW264.7 cells to active osteoclasts. In contrast to RANKL/TGFb that induced expression of several osteoclast-associated genes, RANKL/GDF-15 and RANKL/BMP-7 suppressed it. According to these we document that different members of the TGFb protein family differently regulate signaling in osteoclasts and consequently modulate the final cellular answer.

Links

• GA301/06/0036, research and development project: Name: Úloha vybraných transkripčních faktorů v osteoklastové diferenciační dráze

Investor: Czech Science Foundation, A role of certain transcription factors in the osteoclastic differentiation pathway

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations