Structural Basis of the Preferential Binding for Globo-Series
Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I

J 2008

Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I

BLANCHARD, Bertrand, Alessandra NURISSO, Emilie HOLLWILLE, Cecile TETAUD, Joele WIELLS et. al.

Základní údaje

Originální název

Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I

Název česky

Strukturní základ vazebných preferencí lektinu I z Pseudomonas aeruginosa vůči glykosfingolipidům Globo-serie

Autoři

BLANCHARD, Bertrand (250 Francie), Alessandra NURISSO (380 Itálie), Emilie HOLLWILLE (250 Francie), Cecile TETAUD (50 Bangladéš), Joele WIELLS (250 Francie), Martina POKORNÁ (203 Česká republika), Michaela WIMMEROVÁ (203 Česká republika, garant), Annabelle VARROTTE (250 Francie) a Anne IMBERTY (250 Francie)

Vydání

Journal of Molecular Biology, Amsterdam, Elsevier, 2008, 0022-2836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.146

Kód RIV

RIV/00216224:14310/08:00026609

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000260533800009

Klíčová slova anglicky

pseudomonas aeruginosa; lectin; molecular modeling; structure;glycosphinglipid; oligosaccharides

Štítky

glycosphinglipid, lectin, molecular modeling, oligosaccharides, Pseudomonas aeruginosa, structure

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 6. 2009 12:54, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

ORIG CZ

V originále

The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution.

Česky

Strukturní základ vazebných preferencí lektinu I z Pseudomonas aeruginosa vůči glykosfingolipidům Globo-serie

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

BLANCHARD, Bertrand, Alessandra NURISSO, Emilie HOLLWILLE, Cecile TETAUD, Joele WIELLS, Martina POKORNÁ, Michaela WIMMEROVÁ, Annabelle VARROTTE a Anne IMBERTY. Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I. Journal of Molecular Biology. Amsterdam: Elsevier, 2008, roč. 383, č. 4, s. 837-853. ISSN 0022-2836.

@article{787074,
   author = {Blanchard, Bertrand and Nurisso, Alessandra and Hollwille, Emilie and Tetaud, Cecile and Wiells, Joele and Pokorná, Martina and Wimmerová, Michaela and Varrotte, Annabelle and Imberty, Anne},
   article_location = {Amsterdam},
   article_number = {4},
   keywords = {pseudomonas aeruginosa; lectin; molecular modeling; structure;glycosphinglipid; oligosaccharides},
   language = {eng},
   issn = {0022-2836},
   journal = {Journal of Molecular Biology},
   title = {Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I},
   volume = {383},
   year = {2008}
}

TY  - JOUR
ID  - 787074
AU  - Blanchard, Bertrand - Nurisso, Alessandra - Hollwille, Emilie - Tetaud, Cecile - Wiells, Joele - Pokorná, Martina - Wimmerová, Michaela - Varrotte, Annabelle - Imberty, Anne
PY  - 2008
TI  - Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I
JF  - Journal of Molecular Biology
VL  - 383
IS  - 4
SP  - 837-853
EP  - 837-853
PB  - Elsevier
SN  - 00222836
KW  - pseudomonas aeruginosa
KW  - lectin
KW  - molecular modeling
KW  - structure;glycosphinglipid
KW  - oligosaccharides
N2  - The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution.
ER  -

BLANCHARD, Bertrand, Alessandra NURISSO, Emilie HOLLWILLE, Cecile TETAUD, Joele WIELLS, Martina POKORNÁ, Michaela WIMMEROVÁ, Annabelle VARROTTE a Anne IMBERTY. Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I. \textit{Journal of Molecular Biology}. Amsterdam: Elsevier, 2008, roč.~383, č.~4, s.~837-853. ISSN~0022-2836.

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