Structural Basis of the Preferential Binding for Globo-Series
Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I

J 2008

Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I

BLANCHARD, Bertrand; Alessandra NURISSO; Emilie HOLLWILLE; Cecile TETAUD; Joele WIELLS et. al.

Základní údaje

Originální název

Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I

Název česky

Strukturní základ vazebných preferencí lektinu I z Pseudomonas aeruginosa vůči glykosfingolipidům Globo-serie

Autoři

BLANCHARD, Bertrand (250 Francie); Alessandra NURISSO (380 Itálie); Emilie HOLLWILLE (250 Francie); Cecile TETAUD (50 Bangladéš); Joele WIELLS (250 Francie); Martina POKORNÁ (203 Česká republika); Michaela WIMMEROVÁ (203 Česká republika, garant); Annabelle VARROTTE (250 Francie) a Anne IMBERTY (250 Francie)

Vydání

Journal of Molecular Biology, Amsterdam, Elsevier, 2008, 0022-2836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.146

Kód RIV

RIV/00216224:14310/08:00026609

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000260533800009

Klíčová slova anglicky

pseudomonas aeruginosa; lectin; molecular modeling; structure;glycosphinglipid; oligosaccharides

Štítky

glycosphinglipid, lectin, molecular modeling, oligosaccharides, Pseudomonas aeruginosa, structure

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 6. 2009 12:54, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

ORIG CZ

V originále

The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution.

Česky

Strukturní základ vazebných preferencí lektinu I z Pseudomonas aeruginosa vůči glykosfingolipidům Globo-serie

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

BLANCHARD, Bertrand; Alessandra NURISSO; Emilie HOLLWILLE; Cecile TETAUD; Joele WIELLS; Martina POKORNÁ; Michaela WIMMEROVÁ; Annabelle VARROTTE a Anne IMBERTY. Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I. Journal of Molecular Biology. Amsterdam: Elsevier, 2008, roč. 383, č. 4, s. 837-853. ISSN 0022-2836.

@article{787074,
   author = {Blanchard, Bertrand and Nurisso, Alessandra and Hollwille, Emilie and Tetaud, Cecile and Wiells, Joele and Pokorná, Martina and Wimmerová, Michaela and Varrotte, Annabelle and Imberty, Anne},
   article_location = {Amsterdam},
   article_number = {4},
   keywords = {pseudomonas aeruginosa; lectin; molecular modeling; structure;glycosphinglipid; oligosaccharides},
   language = {eng},
   issn = {0022-2836},
   journal = {Journal of Molecular Biology},
   title = {Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I},
   volume = {383},
   year = {2008}
}

TY  - JOUR
ID  - 787074
AU  - Blanchard, Bertrand - Nurisso, Alessandra - Hollwille, Emilie - Tetaud, Cecile - Wiells, Joele - Pokorná, Martina - Wimmerová, Michaela - Varrotte, Annabelle - Imberty, Anne
PY  - 2008
TI  - Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I
JF  - Journal of Molecular Biology
VL  - 383
IS  - 4
SP  - 837-853
EP  - 837-853
PB  - Elsevier
SN  - 00222836
KW  - pseudomonas aeruginosa
KW  - lectin
KW  - molecular modeling
KW  - structure;glycosphinglipid
KW  - oligosaccharides
N2  - The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution.
ER  -

BLANCHARD, Bertrand; Alessandra NURISSO; Emilie HOLLWILLE; Cecile TETAUD; Joele WIELLS; Martina POKORNÁ; Michaela WIMMEROVÁ; Annabelle VARROTTE a Anne IMBERTY. Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I. \textit{Journal of Molecular Biology}. Amsterdam: Elsevier, 2008, roč.~383, č.~4, s.~837-853. ISSN~0022-2836.

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