2008
Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains
KUNTOVÁ, Lucie, Ivana NEPEJCHALOVÁ, Marian VARGA, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ et. al.Základní údaje
Originální název
Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains
Název česky
Transdukce plazmidů bakteriofágy indukovanými z meticilin rezistentních kmenů Staphylococcus aureus
Autoři
KUNTOVÁ, Lucie (203 Česká republika), Ivana NEPEJCHALOVÁ (203 Česká republika), Marian VARGA (203 Česká republika), Roman PANTŮČEK (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika, garant)
Vydání
Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, s. 49-49, 2008
Nakladatel
Transregional Collaborative Research Center 34
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Kód RIV
RIV/00216224:14310/08:00026646
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Staphylococcus aureus; antimicrobial resistance; horizontal gene transfer; bacteriophages
Příznaky
Mezinárodní význam
Změněno: 31. 10. 2008 14:52, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Our work was focused on generalized transduction of resistance plasmids in MRSA strains. In a set of 66 clinical MRSA strains the plasmid content and lysogenicity were estimated. Lysogenic strains harboring tetracycline and penicillinase plasmids were chosen as the donor strains. The transduction were performed with phage lysates obtained by UV-irradiation of the donor strains. The prophage-less strain 8325-4, restriction-deficient strains SA113 and SA1039, and some clinical MRSA strains were used as recipients. Both penicillinase (30.2 kb) and tetracycline (4.4 kb) resistance plasmids were independently transferred into recipient strains SA113 and 8325-4. The transductants were characterized in detail in respect to transferred plasmids, antibiotic resistance phenotype and lysogenicity with transducing phages. The transducing phage induced from an MRSA strain differs from the well defined transducing phages 11, 80alpha and 53 in all tested characteristics, i.e. in integrase type, in the sequence of portal gene and in restriction endonuclease patterns (HindIII and EcoRI). It is suggested that prophages with transducing ability are responsible for dissemination of resistance determinants in populations of MRSA.
Česky
Our work was focused on generalized transduction of resistance plasmids in MRSA strains. In a set of 66 clinical MRSA strains the plasmid content and lysogenicity were estimated. Lysogenic strains harboring tetracycline and penicillinase plasmids were chosen as the donor strains. The transduction were performed with phage lysates obtained by UV-irradiation of the donor strains. The prophage-less strain 8325-4, restriction-deficient strains SA113 and SA1039, and some clinical MRSA strains were used as recipients. Both penicillinase (30.2 kb) and tetracycline (4.4 kb) resistance plasmids were independently transferred into recipient strains SA113 and 8325-4. The transductants were characterized in detail in respect to transferred plasmids, antibiotic resistance phenotype and lysogenicity with transducing phages. The transducing phage induced from an MRSA strain differs from the well defined transducing phages 11, 80alpha and 53 in all tested characteristics, i.e. in integrase type, in the sequence of portal gene and in restriction endonuclease patterns (HindIII and EcoRI). It is suggested that prophages with transducing ability are responsible for dissemination of resistance determinants in populations of MRSA.
Návaznosti
| MSM0021622415, záměr |
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