2008
Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
RŮŽIČKOVÁ, Vladislava, Pavla HOLOCHOVÁ, Renata KARPÍŠKOVÁ, Roman PANTŮČEK, Markéta POSPÍŠILOVÁ et. al.Základní údaje
Originální název
Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
Název česky
Molekulární typizace enterotoxin H-pozitivních kmenů Staphylococcus aureus izolovaných z pacientů a potravin v České republice
Autoři
RŮŽIČKOVÁ, Vladislava (203 Česká republika, garant), Pavla HOLOCHOVÁ (203 Česká republika), Renata KARPÍŠKOVÁ (203 Česká republika), Roman PANTŮČEK (203 Česká republika), Markéta POSPÍŠILOVÁ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)
Vydání
Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, s. 77-77, 2008
Nakladatel
Transregional Collaborative Research Center 34
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Kód RIV
RIV/00216224:14310/08:00026647
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Staphylococcus aureus; enterotoxin H; staphylococcal food poisoning; molecular diagnostics
Příznaky
Mezinárodní význam
Změněno: 31. 10. 2008 14:52, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
Česky
Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
Návaznosti
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