2008
Genotypic characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic
PANTŮČEK, Roman, Jana KAHÁNKOVÁ, Ivana MACHOVÁ, Petr PETRÁŠ, Lucie KUNTOVÁ et. al.Základní údaje
Originální název
Genotypic characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic
Název česky
Genotypové vlastnosti PVL-pozitivních kmenů Staphylococcus aureus izolovaných v ČR
Autoři
PANTŮČEK, Roman (203 Česká republika), Jana KAHÁNKOVÁ (203 Česká republika, garant), Ivana MACHOVÁ (203 Česká republika), Petr PETRÁŠ (203 Česká republika), Lucie KUNTOVÁ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)
Vydání
Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, s. 74-74, 2008
Nakladatel
Transregional Collaborative Research Center 34
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Kód RIV
RIV/00216224:14310/08:00026649
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Staphylococcus aureus; community acquired MRSA; Panton Valentine leukocidin; molecular diagnostics; mobile genetic elements
Štítky
Příznaky
Mezinárodní význam
Změněno: 7. 1. 2009 22:43, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Phenotypic and genotypic characteristics of PVL positive S. aureus strains isolated from clinical specimens in the Czech Republic between January 2004 and April 2008 are described. Carriage of the lukS-PV and lukF-PV genes, and mecA gene was tested by a multiplex PCR assay. The phenotypic and genotypic methods were used for detection of superantigens (enterotoxin A-J, TSST-1, and exfoliatins A and B). The PVL positive MRSA strains were characterized in detail by PFGE, spa typing, SCCmec typing, arcA gene detection, agr typing, plasmid typing, and prophage typing. The presence of the lukS-PV and lukF-PV genes was detected in 159 (6.8 %) of 2337 S. aureus strains, mainly from lesions, wound infection, skin and soft tissue abscesses, and abscessing pneumonia. Only 21 strains were MRSA, 17 of them were SCCmec type IV, three SCCmec V and one harbored a composite SCCmec with ccrB and ccrC recombinases. Genotypic properties of PVL positive MRSA strains were correlated with the known European caMRSA types. In addition to the predominant spa-type t008 (38 %) exhibiting the characteristics of USA300 clone and t044 (ST80) (14 %) widely disseminated in Central Europe, other spa-types were sporadically represented by t002, t003, t186, t311, t433 and t667 among the caMRSA isolates.
Česky
Phenotypic and genotypic characteristics of PVL positive S. aureus strains isolated from clinical specimens in the Czech Republic between January 2004 and April 2008 are described. Carriage of the lukS-PV and lukF-PV genes, and mecA gene was tested by a multiplex PCR assay. The phenotypic and genotypic methods were used for detection of superantigens (enterotoxin A-J, TSST-1, and exfoliatins A and B). The PVL positive MRSA strains were characterized in detail by PFGE, spa typing, SCCmec typing, arcA gene detection, agr typing, plasmid typing, and prophage typing. The presence of the lukS-PV and lukF-PV genes was detected in 159 (6.8 %) of 2337 S. aureus strains, mainly from lesions, wound infection, skin and soft tissue abscesses, and abscessing pneumonia. Only 21 strains were MRSA, 17 of them were SCCmec type IV, three SCCmec V and one harbored a composite SCCmec with ccrB and ccrC recombinases. Genotypic properties of PVL positive MRSA strains were correlated with the known European caMRSA types. In addition to the predominant spa-type t008 (38 %) exhibiting the characteristics of USA300 clone and t044 (ST80) (14 %) widely disseminated in Central Europe, other spa-types were sporadically represented by t002, t003, t186, t311, t433 and t667 among the caMRSA isolates.
Návaznosti
| MSM0021622415, záměr |
|