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@inproceedings{788643,
author = {Pantůček, Roman and Kahánková, Jana and Machová, Ivana and Petráš, Petr and Kuntová, Lucie and Doškař, Jiří},
address = {Würzburg, Germany},
booktitle = {Pathophysiology of Staphylococci},
edition = {Abstract book},
keywords = {Staphylococcus aureus; community acquired MRSA; Panton Valentine leukocidin; molecular diagnostics; mobile genetic elements},
language = {eng},
location = {Würzburg, Germany},
pages = {74-74},
publisher = {Transregional Collaborative Research Center 34},
title = {Genotypic characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic},
url = {http://www.pathostaph.de/},
year = {2008}
}
TY - JOUR
ID - 788643
AU - Pantůček, Roman - Kahánková, Jana - Machová, Ivana - Petráš, Petr - Kuntová, Lucie - Doškař, Jiří
PY - 2008
TI - Genotypic characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic
PB - Transregional Collaborative Research Center 34
CY - Würzburg, Germany
KW - Staphylococcus aureus
KW - community acquired MRSA
KW - Panton Valentine leukocidin
KW - molecular diagnostics
KW - mobile genetic elements
UR - http://www.pathostaph.de/
N2 - Phenotypic and genotypic characteristics of PVL positive S. aureus strains isolated from clinical specimens in the Czech Republic between January 2004 and April 2008 are described. Carriage of the lukS-PV and lukF-PV genes, and mecA gene was tested by a multiplex PCR assay. The phenotypic and genotypic methods were used for detection of superantigens (enterotoxin A-J, TSST-1, and exfoliatins A and B). The PVL positive MRSA strains were characterized in detail by PFGE, spa typing, SCCmec typing, arcA gene detection, agr typing, plasmid typing, and prophage typing. The presence of the lukS-PV and lukF-PV genes was detected in 159 (6.8 %) of 2337 S. aureus strains, mainly from lesions, wound infection, skin and soft tissue abscesses, and abscessing pneumonia. Only 21 strains were MRSA, 17 of them were SCCmec type IV, three SCCmec V and one harbored a composite SCCmec with ccrB and ccrC recombinases. Genotypic properties of PVL positive MRSA strains were correlated with the known European caMRSA types. In addition to the predominant spa-type t008 (38 %) exhibiting the characteristics of USA300 clone and t044 (ST80) (14 %) widely disseminated in Central Europe, other spa-types were sporadically represented by t002, t003, t186, t311, t433 and t667 among the caMRSA isolates.
ER -
PANTŮČEK, Roman, Jana KAHÁNKOVÁ, Ivana MACHOVÁ, Petr PETRÁŠ, Lucie KUNTOVÁ and Jiří DOŠKAŘ. Genotypic characteristics of Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic. In \textit{Pathophysiology of Staphylococci}. Abstract book. Würzburg, Germany: Transregional Collaborative Research Center 34, 2008, p.~74.
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