An electrochemical detection of metallothioneins at the
zeptomole level in nanolitre volumes

J 2008

An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes

ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ, René KIZEK et. al.

Základní údaje

Originální název

An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes

Název česky

Elektrochemická detekce metalothioneinů v zeptomolárních množstvích v nanolitrových objemech

Autoři

ADAM, Vojtěch (203 Česká republika), Jiří BALOUN (203 Česká republika), Ivo FABRIK (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

Sensors, Basel, Switzerland, MOLECULAR DIVERSITY PRESERVATION INT, 2008, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.870

Kód RIV

RIV/00216224:14310/08:00028434

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000256642400013

Klíčová slova anglicky

metallothionein; voltammetry; biosensor

Štítky

biosensor, metallothionein, Voltammetry

Změněno: 14. 7. 2009 15:19, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. Based on the results obtained it can be concluded that the improved technique can be used to detect a thiolprotein in very low sample volumes and can also prevent interferences during the washing and transferring step.

Česky

Vylepšení metody adsorptivní přenosové rozpouštěcí techniky ve spojení s diferenční pulsní voltametrií ke stanovení thiolových proteinů.

Návaznosti

IAA401990701, projekt VaV

Název: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu

Citovat

ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ a René KIZEK. An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes. Sensors. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč. 8, č. 4, s. 2293-2305, 12 s. ISSN 1424-8220.

@article{791493,
   author = {Adam, Vojtěch and Baloun, Jiří and Fabrik, Ivo and Trnková, Libuše and Kizek, René},
   article_location = {Basel, Switzerland},
   article_number = {4},
   keywords = {metallothionein; voltammetry; biosensor},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes},
   volume = {8},
   year = {2008}
}

TY  - JOUR
ID  - 791493
AU  - Adam, Vojtěch - Baloun, Jiří - Fabrik, Ivo - Trnková, Libuše - Kizek, René
PY  - 2008
TI  - An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
JF  - Sensors
VL  - 8
IS  - 4
SP  - 2293-2305
EP  - 2293-2305
PB  - MOLECULAR DIVERSITY PRESERVATION INT
SN  - 14248220
KW  - metallothionein
KW  - voltammetry
KW  - biosensor
N2  - We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. Based on the results obtained it can be concluded that the improved technique can be used to detect a thiolprotein in very low sample volumes and can also prevent interferences during the washing and transferring step.
ER  -

ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ a René KIZEK. An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes. \textit{Sensors}. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč.~8, č.~4, s.~2293-2305, 12 s. ISSN~1424-8220.

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