| KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA, Grace J. CHAVIS, Libuše TRNKOVÁ, Jan STRNADEL, Vratislav HORÁK a René KIZEK. Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues. Sensors. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč. 8, č. 5, s. 3106-3122, 16 s. ISSN 1424-8220. |
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@article{791494,
author = {Křížková, Soňa and Fabrik, Ivo and Adam, Vojtěch and Kukačka, Jiří and Průša, Richard and Chavis, Grace J. and Trnková, Libuše and Strnadel, Jan and Horák, Vratislav and Kizek, René},
article_location = {Basel, Switzerland},
article_number = {5},
keywords = {melanoma; metallothionein; electrochemistry},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues},
volume = {8},
year = {2008}
}
TY - JOUR
ID - 791494
AU - Křížková, Soňa - Fabrik, Ivo - Adam, Vojtěch - Kukačka, Jiří - Průša, Richard - Chavis, Grace J. - Trnková, Libuše - Strnadel, Jan - Horák, Vratislav - Kizek, René
PY - 2008
TI - Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues
JF - Sensors
VL - 8
IS - 5
SP - 3106-3122
EP - 3106-3122
PB - MOLECULAR DIVERSITY PRESERVATION INT
SN - 14248220
KW - melanoma
KW - metallothionein
KW - electrochemistry
N2 - In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 microM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 micro g of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 +- 0.8 microM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.
ER -
KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA, Grace J. CHAVIS, Libuše TRNKOVÁ, Jan STRNADEL, Vratislav HORÁK a René KIZEK. Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues. \textit{Sensors}. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč.~8, č.~5, s.~3106-3122, 16 s. ISSN~1424-8220.
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