Utilizing of adsorptive transfer stripping technique Brdicka
reaction for determination of metallothioneins level in
melanoma cells, blood serum and tissues

J 2008

Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues

KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA et. al.

Základní údaje

Originální název

Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues

Název česky

Využití adsorptivní přenosové rozpouštěcí techniky Brdičkovy reakce pro stanovení metalothioneinů v melanomových buňkách, krevním séru a tkáních

Autoři

KŘÍŽKOVÁ, Soňa (203 Česká republika), Ivo FABRIK (203 Česká republika), Vojtěch ADAM (203 Česká republika), Jiří KUKAČKA (203 Česká republika), Richard PRŮŠA (203 Česká republika), Grace J. CHAVIS (840 Spojené státy), Libuše TRNKOVÁ (203 Česká republika), Jan STRNADEL (203 Česká republika), Vratislav HORÁK (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

Sensors, Basel, Switzerland, MOLECULAR DIVERSITY PRESERVATION INT, 2008, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.870

Kód RIV

RIV/00216224:14310/08:00028435

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000257248800012

Klíčová slova anglicky

melanoma; metallothionein; electrochemistry

Štítky

electrochemistry, melanoma, metallothionein

Změněno: 14. 7. 2009 15:13, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 microM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 micro g of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 +- 0.8 microM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.

Česky

Využití adsorptivní přenosové rozpouštěcí diferenční pulsní voltametrie ke stanovení metalothioneinů v melanomových buňkách, zvířecích melanomových tkáních a v séru pacientů se zhoubným melanomem.

Návaznosti

IAA401990701, projekt VaV

Název: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu

Citovat

KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA, Grace J. CHAVIS, Libuše TRNKOVÁ, Jan STRNADEL, Vratislav HORÁK a René KIZEK. Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues. Sensors. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč. 8, č. 5, s. 3106-3122, 16 s. ISSN 1424-8220.

@article{791494,
   author = {Křížková, Soňa and Fabrik, Ivo and Adam, Vojtěch and Kukačka, Jiří and Průša, Richard and Chavis, Grace J. and Trnková, Libuše and Strnadel, Jan and Horák, Vratislav and Kizek, René},
   article_location = {Basel, Switzerland},
   article_number = {5},
   keywords = {melanoma; metallothionein; electrochemistry},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues},
   volume = {8},
   year = {2008}
}

TY  - JOUR
ID  - 791494
AU  - Křížková, Soňa - Fabrik, Ivo - Adam, Vojtěch - Kukačka, Jiří - Průša, Richard - Chavis, Grace J. - Trnková, Libuše - Strnadel, Jan - Horák, Vratislav - Kizek, René
PY  - 2008
TI  - Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues
JF  - Sensors
VL  - 8
IS  - 5
SP  - 3106-3122
EP  - 3106-3122
PB  - MOLECULAR DIVERSITY PRESERVATION INT
SN  - 14248220
KW  - melanoma
KW  - metallothionein
KW  - electrochemistry
N2  - In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 microM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 micro g of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 +- 0.8 microM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.
ER  -

KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA, Grace J. CHAVIS, Libuše TRNKOVÁ, Jan STRNADEL, Vratislav HORÁK a René KIZEK. Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues. \textit{Sensors}. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč.~8, č.~5, s.~3106-3122, 16 s. ISSN~1424-8220.

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