Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .

MATULOVÁ, Petra. Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates . 2008.

Základní údaje

• Originální název: Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .
• Název česky: Role SRS2 a Mus81/MMS4 v procesu recombinace a replikace.
• Autoři: MATULOVÁ, Petra (203 Česká republika, garant).
• Vydání: 2008.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Audiovizuální tvorba
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Rakousko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Kód RIV: RIV/00216224:14310/08:00026760
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: DNA repair; homologous recombination; nuclease activity; MUS81; SRS2
• Štítky: DNA repair, HOMOLOGOUS RECOMBINATION, MUS81, nuclease activity, Srs2
• Příznaky: Mezinárodní význam

Anotace

Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific.

Anotace česky

Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific.

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