Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia)

MATULOVÁ, Petra, Kirsti WITTER a Ivan MÍŠEK. Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia). (Microtus agrestis, Rodentia). Taylor and Francis, 2002, roč. 43, č. 43, s. 138–142, 4 s. ISSN 0300-8207.

Základní údaje

Originální název

Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia)

Název česky

Exprese PCNA v zubních základech hraboše mokřadního (Microtus agrestis, Rodentia)

Autoři

MATULOVÁ, Petra (203 Česká republika, garant), Kirsti WITTER (276 Německo) a Ivan MÍŠEK (203 Česká republika)

Vydání

(Microtus agrestis, Rodentia), Taylor and Francis, 2002, 0300-8207

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.646

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Dentition; Odontogenesis; Proliferation Marker; Embryo; Development

Štítky

dentition, development, embryo, Odontogenesis, Proliferation Marker

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNAmarks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.

Česky

Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNAmarks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.