Myeloma Bank - Use in Practice

MATĚJKOVÁ, Eva, Drahomíra KYJOVSKÁ, Jana ČUMOVÁ, Luděk POUR, Petra VIDLÁKOVÁ, Jana MORAVCOVÁ, Marcela RYCOVÁ, Miroslav PENKA and Roman HÁJEK. Myeloma Bank - Use in Practice. In From cell sorting to plasma cell identification and detection of chromosomal aberrations in multiple myeloma and Immunotherapy of cancer: from bench to bedside - Abstracts and Application Manual. 2008. ISBN 978-80-210-4704-4.

Basic information

• Original name: Myeloma Bank - Use in Practice
• Authors: MATĚJKOVÁ, Eva, Drahomíra KYJOVSKÁ, Jana ČUMOVÁ, Luděk POUR, Petra VIDLÁKOVÁ, Jana MORAVCOVÁ, Marcela RYCOVÁ, Miroslav PENKA and Roman HÁJEK.
• Edition: From cell sorting to plasma cell identification and detection of chromosomal aberrations in multiple myeloma and Immunotherapy of cancer: from bench to bedside - Abstracts and Application Manual, 2008.

Other information

• Type of outcome: Conference abstract
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• ISBN: 978-80-210-4704-4

Abstract

Introduction: Long-term storage and archiving of samples in liquid nitrogen is very important for future use, especially for monitoring and precaution experiments. These samples are also considerable for diagnostic and therapeutic usage in myeloma patients. We present the current status in Myeloma Bank from the year 2001 till presence. Methods/Patients: We collect bone marrow samples from patients with new diagnosis of multiple myeloma (MM), with relapse, before therapy and in the phase of maximal therapeutic response. All patients confirmed the informed consent. We isolate mononuclear cells using gradient centrifugation and then we separate CD138+ (rich in myeloma cells) and CD138- cells with MACS (MAgnetic Cell Sorting, Miltenyi Biotec). We assay purity of these fractions – percentage of myeloma cells on cytospin smear and percentage of CD38+ and CD138+ cells with flow cytometry. Native cell fractions are used in following experiments or they are cryoconserved with programmable freezing and then store in liquid nitrogen. Results: From year 2001 till January 2008 we cryoconserved 8 549 samples from myeloma patients in form of CD138+ and CD138- cell fractions, plasma and bone marrow mononuclear cells. From peripheral blood we store serum, plasma and mononuclear cells. Conclusion: Separated CD138+ cells are used in cytogenetic (FISH, CGH), genomic (RT-PCR) and proteomic experiments. These cells are also exploited as a tumor antigen for vaccine preparation on the dentritic cells basis and for cultivation of myeloma specific T-lymphocytes.

Links

• LC06027, research and development project: Name: Univerzitní výzkumné centrum - Česká myelomová skupina (Acronym: LC MGUS)

Investor: Ministry of Education, Youth and Sports of the CR, University Research Centre - Czech Myeloma Group

• 2B06058, research and development project: Name: Bezpečná biotechnologie alotransplantovaných hematopoetických kmenových buněk u vybraných solidních a hematologických nádorů jako adoptivní imunoterapie (Acronym: NPVII11001)

Investor: Ministry of Education, Youth and Sports of the CR