Combined 3-in-1 photometric, fluorimetric and contactless conductometric on-capillary detector for capillary separations: Applicability for simultaneous detection in CE

RYVOLOVÁ, Markéta, Jan PREISLER, Pavel KRÁSENSKÝ, František FORET, Peter HAUSER, Brett PAULL and Miroslav MACKA. Combined 3-in-1 photometric, fluorimetric and contactless conductometric on-capillary detector for capillary separations: Applicability for simultaneous detection in CE. In CECE 2008 5th international interdisciplinary meeting on bioanalysis. 2008. ISBN 978-80-254-3194-8.

Basic information

• Original name: Combined 3-in-1 photometric, fluorimetric and contactless conductometric on-capillary detector for capillary separations: Applicability for simultaneous detection in CE
• Name in Czech: Kombinovaný 3v1 fotometrický, fluorimetrický a bezkontaktní vodivostní detektor pro kapilární separace: Aplikace pro simultání stanovení v CE
• Authors: RYVOLOVÁ, Markéta (203 Czech Republic), Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution), Pavel KRÁSENSKÝ (203 Czech Republic), František FORET (203 Czech Republic), Peter HAUSER (756 Switzerland), Brett PAULL (372 Ireland) and Miroslav MACKA (203 Czech Republic).
• Edition: CECE 2008 5th international interdisciplinary meeting on bioanalysis, 2008.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00058796
• Organization unit: Faculty of Science
• ISBN: 978-80-254-3194-8
• Keywords in English: capillary electrophoresis; simultaneous detection
• Tags: Capillary electrophoresis, simultaneous detection

Abstract

A new design of on-capillary detection combining 3 detection modes - photometric (PD), fluorimetric (FD) and contactless conductivity (C4D) - in one identical point of detection has been recently introduced, tested and characterised. This design allows a combined 3-in-1 detection in capillary separation techniques (CE, CEC, capillary- and nano-LC). A key advantage is the concurrent availability of the three independent on-column detection techniques in the same point, thus avoiding undesirable shifts in separation times caused by multiple parallel detectors. The per-definition concurrent availability of all the 3 detection modes (PD, FD, C4D) offers another unique advantage for the analysis of complex samples, where each of the single detection modes alone might yield an incomplete detection of the present analytes.

Abstract (in Czech)

Práce je zaměřena na demostraci využití kombinovaného detektoru(fotometrický, fluorimetrický a bezkontaktní vodivostní) pro CE. Byly ukázány příklady analýz z oblasti proteomiky nebo analýzy potravin

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations