BUŇKOVÁ, Leona, Pavel ŠVEC, Jiří HALOUZKA, Ivo RUDOLF and Miroslav NĚMEC. Ribotyping and Whole-Cell Protein Analysis of Spirochetes Isolated from Arthropods in the Czech Republic. Annals of Agricultural and Environmental Medicine. 2008, vol. 15, No 2, p. 225-230. ISSN 1232-1966.
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Basic information
Original name Ribotyping and Whole-Cell Protein Analysis of Spirochetes Isolated from Arthropods in the Czech Republic
Name in Czech Ribotypizace a analýzy celkových buněčných proteinů spirochet izolovaných z členovců v České republice
Authors BUŇKOVÁ, Leona (203 Czech Republic), Pavel ŠVEC (203 Czech Republic), Jiří HALOUZKA (203 Czech Republic), Ivo RUDOLF (203 Czech Republic, guarantor) and Miroslav NĚMEC (203 Czech Republic, belonging to the institution).
Edition Annals of Agricultural and Environmental Medicine, 2008, 1232-1966.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Poland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.443
RIV identification code RIV/00216224:14310/08:00024347
Organization unit Faculty of Science
UT WoS 000261767500009
Keywords in English Borrelia burgdorferi sensu lato; Czech Republic; ribotyping; whole-cell protein analysis; taxonomy
Tags Borrelia burgdorferi sensu lato, Czech Republic, ribotyping, taxonomy, whole-cell protein analysis
Tags International impact, Reviewed
Changed by Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 30/7/2013 15:02.
Abstract
In the presented work, six Borrelia burgdorferi sensu lato isolates were recovered from Ixodes ricinus ticks and one strain of spirochete with uncertain taxonomic position isolated from Culex pipiens mosquito collected in the Valtice area (South Moravia, Czech Republic), as well as representative type strains, were further characterized by ribotyping and whole-cell protein fingerprinting. The ribotype patterns obtained with HindIII and EcoRV ribotype patterns showed good correlations with the recently proposed genotypes and grouped each genospecies in a well-separated cluster. The whole-cell protein profiles of genospecies were more heterogenous than the ribotype patterns. The non-identified spirochetal strain was clearly separated from the Borrelia isolates in both methods thus proving their good capacity to characterize Borrelia burgdorferi sensu lato strains.
Abstract (in Czech)
Práce prezentuje výsledky ribotypizace a analýzy celkových buněčných proteinů šesti kmenů Borrelia burgdorferi sensu lato izolovaných z klíštěte Ixodes ricinus.
Links
GA206/03/0726, research and development projectName: Ekologie emergentních mikroorganismů přenosných hematofágními členovci
KJB600930613, research and development projectName: Diverzita kultivovatelných mikroorganizmů v klíšťatech, vektorech patogenů obratlovců
Investor: Academy of Sciences of the Czech Republic, Diversity of cultivable microorganisms of ixodid ticks, recognized vectors of vertebrate pathogens
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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