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@proceedings{807978,
author = {Nováková, Dana and Hubálek, Zdeněk and Sedláček, Ivo},
booktitle = {XII. International Congress of Bacteriology and Applied Microbiology},
keywords = {novel staphylococcus species; species description},
language = {eng},
title = {Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species},
year = {2008}
}
TY - CONF
ID - 807978
AU - Nováková, Dana - Hubálek, Zdeněk - Sedláček, Ivo
PY - 2008
TI - Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species
KW - novel staphylococcus species
KW - species description
N2 - Two strains of Gram-positive cocci isolated from viscera of common voles (Microtus arvalis) were studied by phenotyping and genotyping. Biochemical features were tested by Api Staph kits (bioMérieux) and by conventional tests. Detailed characterization was done by ribotyping, whole-cell protein analysis and rep-PCR using (GTG)5 primer. The phylogenetic position was analysed by partial 16S rRNA sequencing. Analysed strains revealed high mutual similarity of ribotype (EcoRI 88.9% and HindIII 78.6%), protein (95.6%) as well as rep-PCR (98.2%) fingerprints and concurrently were clearly distinct from all validly described staphylococcal taxa. The isolates showed the same partial 16S rRNA sequences, and the maximum sequence similarities were obtained with Staphylococcus muscae (98.3%) and Staphylococcus chromogenes (98.2%). The obtained results proved that the isolates are two different strains representing probably a novel staphylococcal species.
ER -
NOVÁKOVÁ, Dana, Zdeněk HUBÁLEK a Ivo SEDLÁČEK. Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species. In \textit{XII. International Congress of Bacteriology and Applied Microbiology}. 2008.
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