Off-line coupling of capillary electrophoresis to inductively coupled plasma mass spectrometry for elemental speciation

PREISLER, Jan, Ondřej PEŠ, Pavla FOLTYNOVÁ, Viktor KANICKÝ, Pavel KRÁSENSKÝ, Tomáš VACULOVIČ and Radek VYHNÁNEK. Off-line coupling of capillary electrophoresis to inductively coupled plasma mass spectrometry for elemental speciation. In Vitamins 2008 Nutrition and Diagnostics. 2008. ISBN 978-80-7318-708-8.

Basic information

• Original name: Off-line coupling of capillary electrophoresis to inductively coupled plasma mass spectrometry for elemental speciation
• Name in Czech: Off-line spojenní kapilární elektroforézy s hmotnostní spektrometrií indukčně vázaného plazmatu pro elementální speciaci
• Authors: PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Pavla FOLTYNOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Pavel KRÁSENSKÝ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution) and Radek VYHNÁNEK (203 Czech Republic, belonging to the institution).
• Edition: Vitamins 2008 Nutrition and Diagnostics, 2008.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00058802
• Organization unit: Faculty of Science
• ISBN: 978-80-7318-708-8
• Keywords in English: off-line coupling; capillary electrophoresis; inductively coupled plasma mass spectrometry; elemental speciation
• Tags: Capillary electrophoresis, elemental speciation, off-line coupling

Abstract

Forms of metals differentiated by oxidation state or bound ligands differ remarkably in the impact on their physiological function. On-line combination of column separation techniques, such as capillary electrophoresis (CE) or high performance liquid chromatography (HPLC), to inductively coupled plasma mass spectrometry ICP MS belongs to the most powerful tools for elemental speciation. It is usually achieved using nebulizers or direct injection interfaces. When combined with CE, these techniques can reduce separation resolution due to induced liquid flow in the separation column. Here we report the first off-line coupling of capillary electrophoresis with ICP MS, which is based on effluent deposition on a suitable target followed by laser ablation (LA) and transfer into ICP. The technique is compatible with microcolumn separations in terms of preservation both separation resolution and sensitivity. Furthermore, the fractions collected on the target can be archived and analyzed using other detection techniques. Effect of laser fluence and substrate type on the results will be discussed. Use of an absorbing substrate (PETG) allowed reduction of the laser fluence needed for the ablation of the deposited samples. The detection limits of Cr, Cu, Co, Fe, Ni, Sn and Zn deposited on the target were in the subpicomolar range. Chromium was selected as a model analyte for investigation of the ablation process and demonstration of elemental speciation. Two-minute CE - LA ICP MS speciation of 10 pg of Cr(III) and Cr (VI) will be presented; peak halfwidths of as low as 3 seconds were obtained.

Abstract (in Czech)

Viz anglický text.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622411, plan (intention): Name: Studium a aplikace plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Investor: Ministry of Education, Youth and Sports of the CR, Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations