Generation of Specific Antibodies Against Plant Telomere Binding Proteins
| PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra and Jiří FAJKUS. Generation of Specific Antibodies Against Plant Telomere Binding Proteins. In Protein Expression Europe & Antibodies Europe. 2008. |
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| Basic information | |
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| Original name | Generation of Specific Antibodies Against Plant Telomere Binding Proteins |
| Name in Czech | Vývoj specifických protilátek proti rostlinným telomer-vazebným proteinům. |
| Authors | PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra (203 Czech Republic, guarantor) and Jiří FAJKUS (203 Czech Republic). |
| Edition | Protein Expression Europe & Antibodies Europe, 2008. |
| Other information | |
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| Original language | English |
| Type of outcome | Conference abstract |
| Field of Study | Genetics and molecular biology |
| Country of publisher | United States of America |
| Confidentiality degree | is not subject to a state or trade secret |
| RIV identification code | RIV/00216224:14310/08:00025187 |
| Organization unit | Faculty of Science |
| Keywords in English | antibody; AtTRB proteins; plant |
| Tags | antibody, AtTRB proteins, plant |
| Tags | International impact |
| Changed by | Changed by: prof. RNDr. Jiří Fajkus, CSc., učo 28574. Changed: 9/4/2010 12:54. |
| Abstract |
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| AtTRB (Telomeric Repeat Binding) proteins bind plant telomeric sequence (TTTAGGG)n via their Myb domain, similarly to human hTRF1 or hTRF2 (Telomeric Repeat Factor) which bind to human telomeric sequence (TTAGGG)n. In A. thaliana there are five AtTRB homologues. All AtTRBs contain Myb domain at the N-terminus, central H1/5-like domain and coiled-coil domain at the C-terminus. Even though they are similar at AA sequence they partly differ in their chemical properties or in expression levels in different plant compartments. To determine more precisely the binding sequence of AtTRB proteins and their intracellular localization in vivo we need to get specific antibodies for ChIP experiments. We have expressed the least similar peptide sequence of each AtTRB protein that is located in the loop between Myb domain and H1/5 domain in E. coli. |
| Abstract (in Czech) |
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| AtTRB proteiny se vážící se na telomerickou DNA skrz jeich Myb vazebnou doménu, stejně jako lidské hTRF1 a hTRF2. V A. thaliana bylo objeveno 5 homologů AtTRB proteinů. Všechny obsahují C-koncovou Myb doménu a centrální H1/5-like doménu. Vzhledem k vysoké podobnosti všech pěti proteinů bylo nutno při návrhu specifických protilátek vytipovat nejméně homologní oblast. Tato oblast lokalizovaná mezi Myb doménou a h1/5 doménou, byla naklonována do expresních vektorů a exprimována v bakteriálním expresním systému. Takto exprimovanými proteiny byly imunizovány myši, které vytváření monoklonální protilátky, specificky rozeznávající jednotlivé AtTRB proteiny. |
| Links | |
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| GP521/08/P452, research and development project | Name: Charakterizace a funkční diverzita proteinů rodiny SMH, která je specifická pro rostliny |
| Investor: Czech Science Foundation, Characterisation of functional diversity of the plant-specific SMH family of proteins | |
| MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
| Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations | |
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