Detailed Information on Publication Record
2008
Gene Expression differences in bone marrow hematoietic stem cell subpopulation
KOUTNÁ, IrenaBasic information
Original name
Gene Expression differences in bone marrow hematoietic stem cell subpopulation
Name in Czech
Rozdíly genové exprese subpopulací hematopoetických kmenových buněk kostní dřeně
Authors
Edition
3rd ESF functional genomics conference, 2008
Other information
Type of outcome
Konferenční abstrakt
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Informatics
Keywords in English
CD34+ selection, Lin- selection, cDNA microarrays, hematopoietic stem cell
Změněno: 15/1/2009 14:10, doc. RNDr. Irena Koutná, Ph.D.
Abstract
V originále
The gene expression profile of different hematopoietic stem cell (HSCs) subpopulations and the evaluation of correlation with their biological function is still poorly described problem. In order to bring a new information to this very important research area we used cDNA large-scale (19200 ESTs) microarrays technology for expression profiling of three hematopoietic stem cells enriched populations of human bone marrow. In our experiments we evaluated bone marrow HSCs obtained using the following three methods. (i) FACS CD34+ separation, (ii) immunomagnetic CD34+ separation and (iii) Lin- immunomagnetic separation.
Links
| MSM0021622430, plan (intention) |
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| 2B06052, research and development project |
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