| LITERÁKOVÁ, Petra, Jan LOCHMAN, Zbyněk ZDRÁHAL, Zbyněk PROKOP, Vladimír MIKEŠ a Tomáš KAŠPAROVSKÝ. Determination of capsidiol in tobacco cells culture by HPLC. Journal of Chromatographic Science. 2010, roč. 48, č. 6, s. 436-440. ISSN 0021-9665. |
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@article{815033,
author = {Literáková, Petra and Lochman, Jan and Zdráhal, Zbyněk and Prokop, Zbyněk and Mikeš, Vladimír and Kašparovský, Tomáš},
article_number = {6},
keywords = {Capsidiol Tobacco HPLC},
language = {eng},
issn = {0021-9665},
journal = {Journal of Chromatographic Science},
title = {Determination of capsidiol in tobacco cells culture by HPLC},
volume = {48},
year = {2010}
}
TY - JOUR
ID - 815033
AU - Literáková, Petra - Lochman, Jan - Zdráhal, Zbyněk - Prokop, Zbyněk - Mikeš, Vladimír - Kašparovský, Tomáš
PY - 2010
TI - Determination of capsidiol in tobacco cells culture by HPLC
JF - Journal of Chromatographic Science
VL - 48
IS - 6
SP - 436-440
EP - 436-440
SN - 00219665
KW - Capsidiol Tobacco HPLC
N2 - Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. The relative standard deviation across five extractions of known amounts of capsidiol from plant sample was less than 5.1%. The relative standard deviation across five elicitations of cell cultures was less than 5.9%. GC-MS and LC-MS analysis of capsidiol was performed and corresponding MS spectra are presented.
ER -
LITERÁKOVÁ, Petra, Jan LOCHMAN, Zbyněk ZDRÁHAL, Zbyněk PROKOP, Vladimír MIKEŠ a Tomáš KAŠPAROVSKÝ. Determination of capsidiol in tobacco cells culture by HPLC. \textit{Journal of Chromatographic Science}. 2010, roč.~48, č.~6, s.~436-440. ISSN~0021-9665.
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