2010
Determination of capsidiol in tobacco cells culture by HPLC
LITERÁKOVÁ, Petra, Jan LOCHMAN, Zbyněk ZDRÁHAL, Zbyněk PROKOP, Vladimír MIKEŠ et. al.Základní údaje
Originální název
Determination of capsidiol in tobacco cells culture by HPLC
Název česky
Stanovení capsidiolu v kalusových kulturách tabáku pomocí HPLC
Autoři
LITERÁKOVÁ, Petra (203 Česká republika, domácí), Jan LOCHMAN (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Zbyněk PROKOP (203 Česká republika, domácí), Vladimír MIKEŠ (203 Česká republika, domácí) a Tomáš KAŠPAROVSKÝ (203 Česká republika, garant, domácí)
Vydání
Journal of Chromatographic Science, 2010, 0021-9665
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.078
Kód RIV
RIV/00216224:14310/10:00042276
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000280253700003
Klíčová slova anglicky
Capsidiol Tobacco HPLC
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 8. 12. 2015 09:34, prof. Mgr. Tomáš Kašparovský, Ph.D.
V originále
Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. The relative standard deviation across five extractions of known amounts of capsidiol from plant sample was less than 5.1%. The relative standard deviation across five elicitations of cell cultures was less than 5.9%. GC-MS and LC-MS analysis of capsidiol was performed and corresponding MS spectra are presented.
Česky
Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. The relative standard deviation across five extractions of known amounts of capsidiol from plant sample was less than 5.1%. The relative standard deviation across five elicitations of cell cultures was less than 5.9%. GC-MS and LC-MS analysis of capsidiol was performed and corresponding MS spectra are presented.
Návaznosti
| GA522/06/0156, projekt VaV |
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| ME 942, projekt VaV |
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| MSM0021622413, záměr |
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| MSM0021622415, záměr |
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