2009
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
PRAKASH, Rohit, D. SATORY, E. DRAY, A. PAPUSHA, J. SCHELLER et. al.Základní údaje
Originální název
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
Název česky
Kvasinková Mph1 helikáza potlačuje mitotické chromosomové crossovery
Autoři
PRAKASH, Rohit (840 Spojené státy), D. SATORY (840 Spojené státy), E. DRAY (840 Spojené státy), A. PAPUSHA (840 Spojené státy), J. SCHELLER (276 Německo), W. KRAMER (276 Německo), Lumír KREJČÍ (203 Česká republika, garant), Hannah KLEIN (840 Spojené státy), J.E. HABER (840 Spojené státy), Patrick SUNG (840 Spojené státy) a Grzegorz IRA (840 Spojené státy)
Vydání
Genes Dev. 2009, 1549-5477
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 12.075
Kód RIV
RIV/00216224:14310/09:00029171
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000262369700007
Klíčová slova anglicky
Genome instability; recombination; DNA helicase; crossing over; Fanconi anemia
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 1. 7. 2009 08:12, doc. Mgr. Lumír Krejčí, Ph.D.
V originále
Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Overall, the results from our analyses suggest a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks.
Česky
Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Overall, the results from our analyses suggest a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks.
Návaznosti
| GA301/09/1917, projekt VaV |
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| GD203/09/H046, projekt VaV |
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| LC06030, projekt VaV |
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| MSM0021622413, záměr |
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