Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments

PALEČEK, Emil, Marie BRÁZDOVÁ, Hana ČERNOCKÁ, Daniel VLK, Václav BRÁZDA and Bořivoj VOJTĚŠEK. Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments. Oncogene. Basingstoke: Macmillan Press, 1999, vol. 24, No 18, p. 3617-3625. ISSN 0950-9232.

Basic information

Original name

Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments

Authors

PALEČEK, Emil, Marie BRÁZDOVÁ, Hana ČERNOCKÁ, Daniel VLK, Václav BRÁZDA and Bořivoj VOJTĚŠEK

Edition

Oncogene, Basingstoke, Macmillan Press, 1999, 0950-9232

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 6.517

Organization unit

Faculty of Science

Keywords in English

p53; DNA-p53 interaction; scDNA; divalent cation; gel electrophoresis

Tags

divalent cation, DNA-p53 interaction, gel electrophoresis, p53, scDNA

Tags

International impact, Reviewed

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Abstract

V originále

Recently we have shown that wild-type human p53 protein binds preferentially to supercoiled (sc) DNA in vitro in both the presence and absence of the p53 consensus sequence (p53CON). This binding produces a ladder of retarded bands on an agarose gel. The intensity and the number of these hands are decreased by physiological concentrations of zinc ions. At higher zinc concentrations, binding of p53 to scDNA is completely inhibited.