Histone H2AX-dependent GABA(A) receptor regulation of stem cell
proliferation

J 2008

Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation

ANDÄNG, Michael, Jens HJERLING-LEFFLER, Annalena MOLINER, T. Kalle LUNDGREN, Goncalo CASTELO-BRANCO et. al.

Basic information

Original name

Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation

Name in Czech

Regulace proliferace kmenových buněk závislá na histonu H2AX a receptoru pro GABA(A)

Authors

Edition

Nature, 2008, 0028-0836

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 31.434

Organization unit

Faculty of Science

UT WoS

000252554100044

Keywords in English

GABA; STEM CELLS; H2AX

Abstract

ORIG CZ

V originále

Here we provide evidence that an auto/paracrine gama-amino butyric acid (GABA) signaling via GABAA receptors negatively controls embryonic stem (ES) cell and peripheral neural crest stem (NCS) cell proliferation, preimplantation embryonic growth as well as proliferation in the boundary cap stem cell niche, resulting in an attenuation of neuronal progenies from this niche. GABAA receptor activation hyperpolarizes the membrane potential, increase cell volume and cause accumulation of stem cells in the cell cycle S-phase, thereby causing a rapid reduction in cell proliferation. Activated GABAA receptors signal through S-phase checkpoint kinases of the PI3K related kinase (PIKK) family resulting in phosphorylation of the histone variant H2AX, an event that is required for the effects of GABA on stem cell proliferation. This signaling pathway is a critical regulator of proliferation independently of multipotency, apoptosis and overt DNA damage.

In Czech

Práce poskytuje důkazy o tom, že kyselina gamma-amino máselná (GABA) negativně reguluje proliferaci embryonálních kmenových buněk a kmenových buněk neurální lišty, a růst preimplantačního embrya. Aktivace GABA-A receptoru hyperpolarizuje membránový potenciál, zvyšuje buněčný objem a způsobuje akumulaci kmenových buněk v S fázi. GABA-A receptory signálují přes kinázy regulující přechod přes S fázi (kinázy z PIIK rodiny), což vede k fosforylaci histonu H2AX. Fosforylace H2AX je nezbytná pro efekty, která má GABA-A na proliferaci kmenových buněk. Tato signální dráha zásadně reguluje proliferaci, a to nezávisle na multipotenci, apoptóze a celkovém poškození DNA.

Citovat

ANDÄNG, Michael, Jens HJERLING-LEFFLER, Annalena MOLINER, T. Kalle LUNDGREN, Goncalo CASTELO-BRANCO, Evanthia NANOU, Ester POZAS, Vítězslav BRYJA, Sophie HALLIEZ, Hiroshi NISHIMARU, Johannes WILBERTZ, Ernest ARENAS, Martin KOLTZENBURG, Patrick CHARNAY, Abdeljabbar EL MANIRA, Carlos F. IBAÑEZ and Patrik ERNFORS. Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation. Nature. 2008, vol. 451, No 7177, p. 460-464. ISSN 0028-0836.

@article{816467,
   author = {Andäng, Michael and HjerlingandLeffler, Jens and Moliner, Annalena and Lundgren, T. Kalle and CasteloandBranco, Goncalo and Nanou, Evanthia and Pozas, Ester and Bryja, Vítězslav and Halliez, Sophie and Nishimaru, Hiroshi and Wilbertz, Johannes and Arenas, Ernest and Koltzenburg, Martin and Charnay, Patrick and El Manira, Abdeljabbar and Ibañez, Carlos F. and Ernfors, Patrik},
   article_number = {7177},
   keywords = {GABA; STEM CELLS; H2AX},
   language = {eng},
   issn = {0028-0836},
   journal = {Nature},
   title = {Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation},
   volume = {451},
   year = {2008}
}

TY  - JOUR
ID  - 816467
AU  - Andäng, Michael - Hjerling-Leffler, Jens - Moliner, Annalena - Lundgren, T. Kalle - Castelo-Branco, Goncalo - Nanou, Evanthia - Pozas, Ester - Bryja, Vítězslav - Halliez, Sophie - Nishimaru, Hiroshi - Wilbertz, Johannes - Arenas, Ernest - Koltzenburg, Martin - Charnay, Patrick - El Manira, Abdeljabbar - Ibañez, Carlos F. - Ernfors, Patrik
PY  - 2008
TI  - Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation
JF  - Nature
VL  - 451
IS  - 7177
SP  - 460-464
EP  - 460-464
SN  - 00280836
KW  - GABA
KW  - STEM CELLS
KW  - H2AX
N2  - Here we provide evidence that an auto/paracrine gama-amino butyric acid (GABA) signaling via GABAA receptors negatively controls embryonic stem (ES) cell and peripheral neural crest stem (NCS) cell proliferation, preimplantation embryonic growth as well as proliferation in the boundary cap stem cell niche, resulting in an attenuation of neuronal progenies from this niche. GABAA receptor activation hyperpolarizes the membrane potential, increase cell volume and cause accumulation of stem cells in the cell cycle S-phase, thereby causing a rapid reduction in cell proliferation. Activated GABAA receptors signal through S-phase checkpoint kinases of the PI3K related kinase (PIKK) family resulting in phosphorylation of the histone variant H2AX, an event that is required for the effects of GABA on stem cell proliferation. This signaling pathway is a critical regulator of proliferation independently of multipotency, apoptosis and overt DNA damage.
ER  -

ANDÄNG, Michael, Jens HJERLING-LEFFLER, Annalena MOLINER, T. Kalle LUNDGREN, Goncalo CASTELO-BRANCO, Evanthia NANOU, Ester POZAS, Vítězslav BRYJA, Sophie HALLIEZ, Hiroshi NISHIMARU, Johannes WILBERTZ, Ernest ARENAS, Martin KOLTZENBURG, Patrick CHARNAY, Abdeljabbar EL MANIRA, Carlos F. IBAÑEZ and Patrik ERNFORS. Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation. \textit{Nature}. 2008, vol.~451, No~7177, p.~460-464. ISSN~0028-0836.

Detailed Information on Publication Record