| BOUCHAL, Pavel, Theodoros ROUMELIOTIS, Roman HRSTKA, Rudolf NENUTIL, Bořivoj VOJTĚŠEK and Spiros D. GARBIS. Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis. Journal of Proteome Research. Washington, USA: ACS, 2009, vol. 8, No 1, p. 362-373. ISSN 1535-3893. Available from: https://dx.doi.org/10.1021/pr800622b. |
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@article{823185,
author = {Bouchal, Pavel and Roumeliotis, Theodoros and Hrstka, Roman and Nenutil, Rudolf and Vojtěšek, Bořivoj and Garbis, Spiros D.},
article_location = {Washington, USA},
article_number = {1},
doi = {http://dx.doi.org/10.1021/pr800622b},
keywords = {Breast cancer; biomarkers; proteomics; mass spectrometry; liquid chromatography; iTRAQ},
language = {eng},
issn = {1535-3893},
journal = {Journal of Proteome Research},
title = {Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis},
volume = {8},
year = {2009}
}
TY - JOUR
ID - 823185
AU - Bouchal, Pavel - Roumeliotis, Theodoros - Hrstka, Roman - Nenutil, Rudolf - Vojtěšek, Bořivoj - Garbis, Spiros D.
PY - 2009
TI - Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis
JF - Journal of Proteome Research
VL - 8
IS - 1
SP - 362-373
EP - 362-373
PB - ACS
SN - 15353893
KW - Breast cancer
KW - biomarkers
KW - proteomics
KW - mass spectrometry
KW - liquid chromatography
KW - iTRAQ
N2 - The present pilot study constitutes a proof-of-principle in the use of a quantitative LC-MS/MS based proteomic method for the comparative analysis of representative low-grade breast primary tumor tissues with and without metastases and metastasis in lymph node relative to the nonmetastatic tumor type. The study method incorporated iTRAQ stable isotope labeling, two-dimensional liquid chromatography, nanoelectrospray ionization and high resolution tandem mass spectrometry using the hybrid QqTOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were (1) to define the protein spectrum obtainable using this approach, and (2) to highlight potential candidates for verification and validation studies focused on biomarkers involved in metastatic processes in breast cancer. The study resulted in the reproducible identification of 605 nonredundant proteins.
ER -
BOUCHAL, Pavel, Theodoros ROUMELIOTIS, Roman HRSTKA, Rudolf NENUTIL, Bořivoj VOJTĚŠEK and Spiros D. GARBIS. Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis. \textit{Journal of Proteome Research}. Washington, USA: ACS, 2009, vol.~8, No~1, p.~362-373. ISSN~1535-3893. Available from: https://dx.doi.org/10.1021/pr800622b.
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