KAHÁNKOVÁ, Jana, Alena ŠPANOVÁ, Roman PANTŮČEK, Daniel HORÁK, Jiří DOŠKAŘ a Bohuslav RITTICH. Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2009, roč. 877, č. 7, s. 599-602. ISSN 1570-0232. |
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@article{823220, author = {Kahánková, Jana and Španová, Alena and Pantůček, Roman and Horák, Daniel and Doškař, Jiří and Rittich, Bohuslav}, article_location = {Amsterdam, The Netherlands}, article_number = {7}, keywords = {Magnetic microspheres; P(HEMA-co-EDMA); Staphylococcus aureus; Bacteriophage DNA; Polymerase chain reaction (PCR)}, language = {eng}, issn = {1570-0232}, journal = {Journal of Chromatography B}, title = {Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres}, url = {http://linkinghub.elsevier.com/retrieve/pii/S1570023209000233}, volume = {877}, year = {2009} }
TY - JOUR ID - 823220 AU - Kahánková, Jana - Španová, Alena - Pantůček, Roman - Horák, Daniel - Doškař, Jiří - Rittich, Bohuslav PY - 2009 TI - Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres JF - Journal of Chromatography B VL - 877 IS - 7 SP - 599-602 EP - 599-602 PB - Elsevier SN - 15700232 KW - Magnetic microspheres KW - P(HEMA-co-EDMA) KW - Staphylococcus aureus KW - Bacteriophage DNA KW - Polymerase chain reaction (PCR) UR - http://linkinghub.elsevier.com/retrieve/pii/S1570023209000233 N2 - Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection. ER -
KAHÁNKOVÁ, Jana, Alena ŠPANOVÁ, Roman PANTŮČEK, Daniel HORÁK, Jiří DOŠKAŘ a Bohuslav RITTICH. Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2009, roč.~877, č.~7, s.~599-602. ISSN~1570-0232.
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