Detoxification and oxidative stress responses along with
microcystins accumulation in Japanese quail exposed to
cyanobacterial biomass.

J 2008

Detoxification and oxidative stress responses along with microcystins accumulation in Japanese quail exposed to cyanobacterial biomass.

PAŠKOVÁ, Veronika, Ondřej ADAMOVSKÝ, Jiří PIKULA, Blanka SKOČOVSKÁ, Hana BAND'OUCHOVÁ et. al.

Základní údaje

Originální název

Detoxification and oxidative stress responses along with microcystins accumulation in Japanese quail exposed to cyanobacterial biomass.

Název česky

Detoxifikace, oxidativní stres a akumulace microcystinů v křepeloe japonské exponované sinicovou biomasou.

Autoři

PAŠKOVÁ, Veronika (203 Česká republika), Ondřej ADAMOVSKÝ (203 Česká republika), Jiří PIKULA (203 Česká republika), Blanka SKOČOVSKÁ (203 Česká republika), Hana BAND'OUCHOVÁ (203 Česká republika), Jana HORÁKOVÁ (203 Česká republika), Pavel BABICA (203 Česká republika), Blahoslav MARŠÁLEK (203 Česká republika) a Klára HILSCHEROVÁ (203 Česká republika, garant)

Vydání

Science ot the Total Environment, Amsterdam, Netherlands, Elsevier, 2008, 0048-9697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.579

Kód RIV

RIV/00216224:14310/08:00035351

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000256979900005

Klíčová slova česky

křepelka; sinice; oxidativní stres; microcystin; detoxifikace

Klíčová slova anglicky

quail; cyanobacteria; oxidative stress; microcystin; detoxification

Štítky

cyanobacteria, detoxification, microcystin, oxidative stress, quail

Změněno: 9. 4. 2009 13:21, doc. Mgr. Klára Hilscherová, Ph.D.

Anotace

ORIG CZ

V originále

The cyanobacterial exposure has been implicated in mass mortalities of wild birds, but information on the actual effects of cyanobacteria on birds in controlled studies is missing. Effects on detoxification and antioxidant parameters as well as bioaccumulation of microcystins (MCs) were studied in birds after sub-lethal exposure to natural cyanobacterial biomass. Four treatment groups of model species Japanese quail (Coturnix coturnix japonica) were exposed to controlled doses of cyanobacterial bloom during acute (10 days) and subchronic (30 days) experiment. Significant accumulation of MCs was observed in the liver for both test durations and slight accumulation also in themuscles of the highest treatment group from acute test. The greatest accumulationwas observed in the liver of the highest treatment group in the acute test reaching average concentration of 43.7 ngMCs/g freshweight.The parameters of detoxification metabolism and oxidative stress were studied in the liver, heart and brain. The cyanobacterial exposure caused an increase of activity of cytochrome P-450-dependent 7-ethoxyresorufin O-deethylase representing the activation phase of detoxification metabolism. Also the conjugation phase of detoxification, namely the activity of glutathione-S-transferase, was altered. Cyanobacterial exposure alsomodulated oxidative stress responses including the level of glutathione and activities of glutathione-related enzymes and caused increase in lipid peroxidation. The overall pattern of detoxification parameters and oxidative stress responses clearly separated the control and the lowest exposure group from all the higher exposed groups. This is the first controlled study documenting the induction of oxidative stress along with MCs accumulation in birds exposed to natural cyanobacterial biomass. The data also suggest that increased activities of detoxification enzymes could lead to greater biotransformation and elimination of the MCs at the longer exposure time.

Česky

Studie ovlivnění detoxifikace, parametrů oxidativního stresu a akumulace microcystinů v křepelce japonské exponované sinicovou biomasou.

Citovat

PAŠKOVÁ, Veronika, Ondřej ADAMOVSKÝ, Jiří PIKULA, Blanka SKOČOVSKÁ, Hana BAND'OUCHOVÁ, Jana HORÁKOVÁ, Pavel BABICA, Blahoslav MARŠÁLEK a Klára HILSCHEROVÁ. Detoxification and oxidative stress responses along with microcystins accumulation in Japanese quail exposed to cyanobacterial biomass. Science ot the Total Environment. Amsterdam, Netherlands: Elsevier, 2008, roč. 398, 1-3, s. 34-47. ISSN 0048-9697.

@article{827605,
   author = {Pašková, Veronika and Adamovský, Ondřej and Pikula, Jiří and Skočovská, Blanka and Band'ouchová, Hana and Horáková, Jana and Babica, Pavel and Maršálek, Blahoslav and Hilscherová, Klára},
   article_location = {Amsterdam, Netherlands},
   article_number = {1-3},
   keywords = {quail; cyanobacteria; oxidative stress; microcystin; detoxification},
   language = {eng},
   issn = {0048-9697},
   journal = {Science ot the Total Environment},
   title = {Detoxification and oxidative stress responses along with microcystins accumulation in Japanese quail exposed to cyanobacterial biomass.},
   volume = {398},
   year = {2008}
}

TY  - JOUR
ID  - 827605
AU  - Pašková, Veronika - Adamovský, Ondřej - Pikula, Jiří - Skočovská, Blanka - Band'ouchová, Hana - Horáková, Jana - Babica, Pavel - Maršálek, Blahoslav - Hilscherová, Klára
PY  - 2008
TI  - Detoxification and oxidative stress responses along with microcystins accumulation in Japanese quail exposed to cyanobacterial biomass.
JF  - Science ot the Total Environment
VL  - 398
IS  - 1-3
SP  - 34-47
EP  - 34-47
PB  - Elsevier
SN  - 00489697
KW  - quail
KW  - cyanobacteria
KW  - oxidative stress
KW  - microcystin
KW  - detoxification
N2  - The cyanobacterial exposure has been implicated in mass mortalities of wild birds, but information on the actual effects of cyanobacteria on birds in controlled studies is missing. Effects on detoxification and antioxidant parameters as well as bioaccumulation of microcystins (MCs) were studied in birds after sub-lethal exposure to natural cyanobacterial biomass. Four treatment groups of model species Japanese quail (Coturnix coturnix japonica) were exposed to controlled doses of cyanobacterial bloom during acute (10 days) and subchronic (30 days) experiment. Significant accumulation of MCs was observed in the liver for both test durations and slight accumulation also in themuscles of the highest treatment group from acute test. The greatest accumulationwas observed in the liver of the highest treatment group in the acute test reaching average concentration of 43.7 ngMCs/g freshweight.The parameters of detoxification metabolism and oxidative stress were studied in the liver, heart and brain. The cyanobacterial exposure caused an increase of activity of cytochrome P-450-dependent 7-ethoxyresorufin O-deethylase representing the activation phase of detoxification metabolism. Also the conjugation phase of detoxification, namely the activity of glutathione-S-transferase, was altered. Cyanobacterial exposure alsomodulated oxidative stress responses including the level of glutathione and activities of glutathione-related enzymes and caused increase in lipid peroxidation. The overall pattern of detoxification parameters and oxidative stress responses clearly separated the control and the lowest exposure group from all the higher exposed groups. This is the first controlled study documenting the induction of oxidative stress along with MCs accumulation in birds exposed to natural cyanobacterial biomass. The data also suggest that increased activities of detoxification enzymes could lead to greater biotransformation and elimination of the MCs at the longer exposure time.
ER  -

PAŠKOVÁ, Veronika, Ondřej ADAMOVSKÝ, Jiří PIKULA, Blanka SKOČOVSKÁ, Hana BAND'OUCHOVÁ, Jana HORÁKOVÁ, Pavel BABICA, Blahoslav MARŠÁLEK a Klára HILSCHEROVÁ. Detoxification and oxidative stress responses along with microcystins accumulation in Japanese quail exposed to cyanobacterial biomass. \textit{Science ot the Total Environment}. Amsterdam, Netherlands: Elsevier, 2008, roč.~398, 1-3, s.~34-47. ISSN~0048-9697.

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