| KUBÍKOVÁ, Iva, Hana KONEČNÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL, Pavel ŘEHULKA, Hana HŘÍBKOVÁ, Helena ŘEHULKOVÁ, Aleš HAMPL, Josef CHMELÍK and Petr DVOŘÁK. Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin. Cytotherapy. United Kingdom, 2009, vol. 11, No 3, p. 330-340. ISSN 1465-3249. Available from: https://dx.doi.org/10.1080/14653240802595531. |
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@article{828562,
author = {Kubíková, Iva and Konečná, Hana and Šedo, Ondrej and Zdráhal, Zbyněk and Řehulka, Pavel and Hříbková, Hana and Řehulková, Helena and Hampl, Aleš and Chmelík, Josef and Dvořák, Petr},
article_location = {United Kingdom},
article_number = {3},
doi = {http://dx.doi.org/10.1080/14653240802595531},
keywords = {human embryonic stem cell; hESC},
language = {eng},
issn = {1465-3249},
journal = {Cytotherapy},
title = {Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin},
url = {https://www.sciencedirect.com/science/article/pii/S1465324909702804?via%3Dihub},
volume = {11},
year = {2009}
}
TY - JOUR
ID - 828562
AU - Kubíková, Iva - Konečná, Hana - Šedo, Ondrej - Zdráhal, Zbyněk - Řehulka, Pavel - Hříbková, Hana - Řehulková, Helena - Hampl, Aleš - Chmelík, Josef - Dvořák, Petr
PY - 2009
TI - Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin
JF - Cytotherapy
VL - 11
IS - 3
SP - 330-340
EP - 330-340
SN - 14653249
KW - human embryonic stem cell
KW - hESC
UR - https://www.sciencedirect.com/science/article/pii/S1465324909702804?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S1465324909702804?via%3Dihub
N2 - Microvesicles (MVs) serve as mediators of cell-to-cell communication. However, they may also contain immunogenic domains or infectious particles acquired from xenogenic components of the culture milieu. Therefore, MVs represent a potential risk for the clinical application of cell therapy. We tested the ability of human embryonic stem cells (hESCs) to produce MVs. We found that hESCs are potent producers of MVs and we identified 5 unique protein species that are known to be highly expressed in invasive cancers and that participate in cellular activation, metastasis, and inhibition of apoptosis. Moreover, we found that hESC-derived MVs contain immunogenic agents apolipoprotein and transferrin, as well as mouse retroviral Gag protein. These findings highlight a potential danger of the clinical use of hESCs that have been previously exposed to animal proteins or cells.
ER -
KUBÍKOVÁ, Iva, Hana KONEČNÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL, Pavel ŘEHULKA, Hana HŘÍBKOVÁ, Helena ŘEHULKOVÁ, Aleš HAMPL, Josef CHMELÍK and Petr DVOŘÁK. Proteomic profiling of human embryonic stem cell-derived microvesicles reveals a risk of transfer of proteins of bovine and mouse origin. \textit{Cytotherapy}. United Kingdom, 2009, vol.~11, No~3, p.~330-340. ISSN~1465-3249. Available from: https://dx.doi.org/10.1080/14653240802595531.
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