| MUSILOVÁ, Jindra, Vojtěch SEDLÁČEK, Igor KUČERA a Zdeněk GLATZ. Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2009, roč. 32, č. 14, s. 2416-2420. ISSN 1615-9306. |
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@article{831359,
author = {Musilová, Jindra and Sedláček, Vojtěch and Kučera, Igor and Glatz, Zdeněk},
article_location = {Weinheim},
article_number = {14},
keywords = {Adenine Nucleotides and Coenzymes; Capillary zone electrophoresis; Field enhanced sample stacking; Metabolomics; Paracoccus denitrificans},
language = {eng},
issn = {1615-9306},
journal = {Journal of Separation Science},
title = {Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans},
volume = {32},
year = {2009}
}
TY - JOUR
ID - 831359
AU - Musilová, Jindra - Sedláček, Vojtěch - Kučera, Igor - Glatz, Zdeněk
PY - 2009
TI - Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans
JF - Journal of Separation Science
VL - 32
IS - 14
SP - 2416-2420
EP - 2416-2420
PB - WILEY-VCH Verlag GmbH & Co.
SN - 16159306
KW - Adenine Nucleotides and Coenzymes
KW - Capillary zone electrophoresis
KW - Field enhanced sample stacking
KW - Metabolomics
KW - Paracoccus denitrificans
N2 - The main aim of this work was to demonstrate the applicability of capillary zone electrophoresis in combination with field enhanced sample stacking in targeted metabolome analyses of adenine nucleotides - AMP, ADP, ATP, coenzymes NAD+, NADP+ and their reduced forms in Paracoccus denitrificans. 100 mM sodium carbonate/hydrogencarbonate buffer (pH 9.6) with the addition of beta-cyclodextrin at a concentration of 10 mM was found to be an effective background electrolyte for their separation within 20 minutes. Besides this, special attention was paid to the development of the procedure for the extraction of specific metabolites from the bacterium Paracoccus denitrificans. This procedure was not only optimised to achieve the highest metabolite yields but also to obtain a sample that was fully compatible with the on-line preconcetration strategy used. The developed methodology was finally applied in a study of the bacterium P. denitrificans at various stages of the active respiratory chain.
ER -
MUSILOVÁ, Jindra, Vojtěch SEDLÁČEK, Igor KUČERA a Zdeněk GLATZ. Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2009, roč.~32, č.~14, s.~2416-2420. ISSN~1615-9306.
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