Homocysteine and thiols in plasma and follicular fluid during FSH ovarian stimulation

KUBEŠOVÁ, Jitka, Jaroslava TALLOVÁ, Igor CRHA and Jiří JARKOVSKÝ. Homocysteine and thiols in plasma and follicular fluid during FSH ovarian stimulation. In Scripta Medica, Meetings and abstracts of the Czechoslovak Biological Society in 2008 year. 2008.

Basic information

• Original name: Homocysteine and thiols in plasma and follicular fluid during FSH ovarian stimulation
• Name in Czech: Homocystein a thioly v krvi a folikulární tekutině při ovariální stimulaci FSH
• Authors: KUBEŠOVÁ, Jitka, Jaroslava TALLOVÁ, Igor CRHA and Jiří JARKOVSKÝ.
• Edition: Scripta Medica, Meetings and abstracts of the Czechoslovak Biological Society in 2008 year, 2008.

Other information

• Type of outcome: Conference abstract
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords (in Czech): homocystein; cystein; cysteinylglycin; glutathion; těhotenství; stimulace; folikulární tekutina; HPLC
• Keywords in English: homocysteine; cysteine; cysteinylglycine; glutathione; pregnancy; stimulation; follicular fluid; HPLC
• Tags: cysteine, cysteinylglycine, follicular fluid, Glutathione, homocysteine, HPLC, pregnancy, stimulation

Abstract

Elevated plasma homocysteine is an independent risk factor for the development of many pathological states such as atherosclerosis, thromboembolic and neurodegenerative disorders, particularly neural tube defects and late pregnancy complications (pre-eclampsia, intrauterine grow retardation, pre-term birth and intrauterine fetal death and abruptio placentae). The metabolism of homocysteine is closely associated with related aminothiols – cysteine, cysteinylglycine and glutathione. The aim of the study was to measure and determine the concentrations of homocysteine (Hcy), cysteine (Cys), glutathione (GSH) in plasma and follicular fluid of women undergoing IVF and to analyse possible correlations. Fourteen women undergoing IVF/ICSI were enrolled in this study. The concentrations of Hcy and Cys in follicular fluid correlated with the concentrations in plasma before stimulation and after oocyte retrieval. Significant differences in the concentrations of Hcy, Cys and GSH in follicular fluid with and without oocytes were not confirmed. The concentrations of Hcy and Cys were significantly lower together with higher 17b-estradiol after stimulation in plasma. A possible correlation between the number of oocytes, embryos, pregnancies and the levels of Hcy, Cys and GSH was not found.

Abstract (in Czech)

Zvýšená hladina homocysteinu je rizikový faktor pro vznik mnoha patologických stavů jako je ateroskleróza, tromobembolické a neurodegenerativní poruchy, defekty neurální trubice a komplikace v těhotenství. Metabolismus homocysteinu úzce souvisí s dalšími aminothioly - cysteinem, cysteinylglycinem a glutathionem. Cílem této práce bylo zjistit hladiny homocysteinu (Hcy), cysteinu (Cys) a glutathionu (GSH) v plazmě a folikulární tekutině u žen podstupujících IVF a analyzovat možné korelace. Studie prokázala signifikantní pokles hladin Hcy a Cys v krvi při ovariální stimulaci za vzestupu hladin 17-beta estradiolu. Hodnoty Hcy a Cys ve folikulární tekutině korelovaly s koncentrací v séru. Souvislost mezi počtem získaných oocytů, embryí, dosažením těhotenství a hladinou Hcy, Cys a GSH nebyla prokázána.

Abstract (in English)

Elevated plasma homocysteine is an independent risk factor for the development of many pathological states such as atherosclerosis, thromboembolic and neurodegenerative disorders, particularly neural tube defects and late pregnancy complications (pre-eclampsia, intrauterine grow retardation, pre-term birth and intrauterine fetal death and abruptio placentae). The metabolism of homocysteine is closely associated with related aminothiols – cysteine, cysteinylglycine and glutathione. The aim of the study was to measure and determine the concentrations of homocysteine (Hcy), cysteine (Cys), glutathione (GSH) in plasma and follicular fluid of women undergoing IVF and to analyse possible correlations. Fourteen women undergoing IVF/ICSI were enrolled in this study. The concentrations of Hcy and Cys in follicular fluid correlated with the concentrations in plasma before stimulation and after oocyte retrieval. Significant differences in the concentrations of Hcy, Cys and GSH in follicular fluid with and without oocytes were not confirmed. The concentrations of Hcy and Cys were significantly lower together with higher 17b-estradiol after stimulation in plasma. A possible correlation between the number of oocytes, embryos, pregnancies and the levels of Hcy, Cys and GSH was not found.