KOMÁREK, Jan, Nikola KOSTLÁNOVÁ, Annabelle VARROT, Anne IMBERTY and Michaela WIMMEROVÁ. Structural and functional characterization of lectin AFL1 from Aspergillus fumigatus. In XIII. Setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita, 2009, p. 69-70. ISBN 978-80-210-4830-0.
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Basic information
Original name Structural and functional characterization of lectin AFL1 from Aspergillus fumigatus
Name in Czech Strukturní a funkční charakterizace lektinu AFL1 z Aspergillus fumigatus
Authors KOMÁREK, Jan, Nikola KOSTLÁNOVÁ, Annabelle VARROT, Anne IMBERTY and Michaela WIMMEROVÁ.
Edition Brno, XIII. Setkání biochemiků a molekulárních biologů, p. 69-70, 2 pp. 2009.
Publisher Masarykova univerzita
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Organization unit Faculty of Science
ISBN 978-80-210-4830-0
Keywords in English lectin Aspergillus fumigatus
Tags lectin Aspergillus fumigatus
Changed by Changed by: prof. RNDr. Michaela Wimmerová, Ph.D., učo 854. Changed: 9/4/2010 09:42.
Abstract
This study deals with structural and functional characterization of 34.6 kDa lectin AFL1 from ascomycete Aspergillus fumigatus. AFL1 was expressed in a recombinant form and purified by FPLC on mannose-agarose column. Binding properties of AFL1 were determined by SPR and ITC. Several promising crystals for X-ray diffraction have been obtained using hanging drop vapour diffusion technique combined with streak seeding technique.
Abstract (in Czech)
Tato práce se zabývá strukturní a funkční charakterizací 34,6 kDa lektinu AFL1 z patogenní plísně Aspergillus fumigatus. AFL1 byl připraven v rekombinantní formě a purifikován pomocí FPLC na mannosa-agarosové koloně. Vazebné vlastnosti AFL1 byly měřeny pomocí SPR a ITC. Pomocí metody visící kapky kombinované s technikou streak seedingu bylo získáno několik nadějných krystalů pro rentgenstrukturní analýzu.
Links
GA303/09/1168, research and development projectName: Lektiny z lidských patogenů - struktura, funkce, inženýrství
Investor: Czech Science Foundation, Lectins from human pathogens - structure, function, engineering
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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