Expression of p53, cyclin D1 and EGFR correlates with histological grade of adult soft tissue sarcomas: a study on tissue microarrays

VESELÝ, Karel, Michal JURAJDA, Rudolf NENUTIL and Marcela VESELÁ. Expression of p53, cyclin D1 and EGFR correlates with histological grade of adult soft tissue sarcomas: a study on tissue microarrays. Neoplasma. Bratislava, Slovensko: AEPress, s.r.o., 2009, vol. 56, No 3, p. 239-244. ISSN 0028-2685.

Basic information

• Original name: Expression of p53, cyclin D1 and EGFR correlates with histological grade of adult soft tissue sarcomas: a study on tissue microarrays
• Name in Czech: Exprese proteinu p 53, cyklinu D1 a EGFR koreluje s histlogickým grade sarkomů u dospělých
• Authors: VESELÝ, Karel, Michal JURAJDA, Rudolf NENUTIL and Marcela VESELÁ.
• Edition: Neoplasma, Bratislava, Slovensko, AEPress, s.r.o. 2009, 0028-2685.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.192
• Organization unit: Faculty of Medicine
• UT WoS: 000265622200009
• Keywords (in Czech): Immunohistochemie; sarkom měkkých tkání; Ki-67; p53; cyklin D1; EGFR; histologický grade; tkáňové mikroereje
• Keywords in English: Immunohistochemistry; soft tissue sarcoma; Ki-67; p53; cyclin D1; EGFR; histological grade; tissue microarrays
• Tags: cyclin D1, EGFR, histological grade, Immunohistochemistry, Ki-67, p53, soft tissue sarcoma, tissue microarrays
• Tags: Reviewed

Abstract

Soft tissue sarcomas (STSs) are rare heterogeneous tumors with variable clinical course and outcome. The management of STSs depends upon the accurate histopathological diagnosis and assessing their histological grade. Currently, core needle biopsies are becoming increasingly popular for diagnosing STSs but value of histological grading is limited from this type of specimens. To evaluate the immunohistochemical expression of p53, mdm-2, cyclin D1, p16, nm23, EGFR and Ki-67 labelling index in adult STSs patients and their association with histological grade of STSs, we analysed 101 primary untreated STSs of the limbs and trunk using the tissue microarray technique on formalin-fixed, paraffin-embedded tissue samples. The cases consisted of 15 G1, 28 G2 and 58 G2 sarcomas. Ki-67 labelling index (LI) was calculated from whole block sections for the possibility to select the most proliferative regions. The LI ranged from 1.26 to 75.5% (median 26.7%) and strongly correlated with the mitotic count (p rs for adult patients with STSs and may assist in establishment of the histological grade in STSs.

Abstract (in Czech)

Sarkomy měkkých tkání (STSs) jsou vzácnou heterogenní skupinou nádorů s variabilním klinickým průběhem. The management of STSs depends upon the accurate histopathological diagnosis and assessing their histological grade. Currently, core needle biopsies are becoming increasingly popular for diagnosing STSs but value of histological grading is limited from this type of specimens. To evaluate the immunohistochemical expression of p53, mdm-2, cyclin D1, p16, nm23, EGFR and Ki-67 labelling index in adult STSs patients and their association with histological grade of STSs, we analysed 101 primary untreated STSs of the limbs and trunk using the tissue microarray technique on formalin-fixed, paraffin-embedded tissue samples. The cases consisted of 15 G1, 28 G2 and 58 G2 sarcomas. Ki-67 labelling index (LI) was calculated from whole block sections for the possibility to select the most proliferative regions. The LI ranged from 1.26 to 75.5% (median 26.7%) and strongly correlated with the mitotic count (p rs for adult patients with STSs and may assist in establishment of the histological grade in STSs.