Expression of p53, cyclin D1 and EGFR correlates with histological grade of adult soft tissue sarcomas: a study on tissue microarrays

VESELÝ, Karel, Michal JURAJDA, Rudolf NENUTIL and Marcela VESELÁ. Expression of p53, cyclin D1 and EGFR correlates with histological grade of adult soft tissue sarcomas: a study on tissue microarrays. Neoplasma. Bratislava, Slovensko: AEPress, s.r.o., 2009, vol. 56, No 3, p. 239-244. ISSN 0028-2685.

Basic information

Original name

Expression of p53, cyclin D1 and EGFR correlates with histological grade of adult soft tissue sarcomas: a study on tissue microarrays

Name in Czech

Exprese proteinu p 53, cyklinu D1 a EGFR koreluje s histlogickým grade sarkomů u dospělých

Authors

VESELÝ, Karel, Michal JURAJDA, Rudolf NENUTIL and Marcela VESELÁ

Edition

Neoplasma, Bratislava, Slovensko, AEPress, s.r.o. 2009, 0028-2685

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.192

Organization unit

Faculty of Medicine

UT WoS

000265622200009

Keywords (in Czech)

Immunohistochemie; sarkom měkkých tkání; Ki-67; p53; cyklin D1; EGFR; histologický grade; tkáňové mikroereje

Keywords in English

Immunohistochemistry; soft tissue sarcoma; Ki-67; p53; cyclin D1; EGFR; histological grade; tissue microarrays

Tags

cyclin D1, EGFR, histological grade, Immunohistochemistry, Ki-67, p53, soft tissue sarcoma, tissue microarrays

Tags

Reviewed

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Abstract

V originále

Soft tissue sarcomas (STSs) are rare heterogeneous tumors with variable clinical course and outcome. The management of STSs depends upon the accurate histopathological diagnosis and assessing their histological grade. Currently, core needle biopsies are becoming increasingly popular for diagnosing STSs but value of histological grading is limited from this type of specimens. To evaluate the immunohistochemical expression of p53, mdm-2, cyclin D1, p16, nm23, EGFR and Ki-67 labelling index in adult STSs patients and their association with histological grade of STSs, we analysed 101 primary untreated STSs of the limbs and trunk using the tissue microarray technique on formalin-fixed, paraffin-embedded tissue samples. The cases consisted of 15 G1, 28 G2 and 58 G2 sarcomas. Ki-67 labelling index (LI) was calculated from whole block sections for the possibility to select the most proliferative regions. The LI ranged from 1.26 to 75.5% (median 26.7%) and strongly correlated with the mitotic count (p rs for adult patients with STSs and may assist in establishment of the histological grade in STSs.

In Czech

Sarkomy měkkých tkání (STSs) jsou vzácnou heterogenní skupinou nádorů s variabilním klinickým průběhem. The management of STSs depends upon the accurate histopathological diagnosis and assessing their histological grade. Currently, core needle biopsies are becoming increasingly popular for diagnosing STSs but value of histological grading is limited from this type of specimens. To evaluate the immunohistochemical expression of p53, mdm-2, cyclin D1, p16, nm23, EGFR and Ki-67 labelling index in adult STSs patients and their association with histological grade of STSs, we analysed 101 primary untreated STSs of the limbs and trunk using the tissue microarray technique on formalin-fixed, paraffin-embedded tissue samples. The cases consisted of 15 G1, 28 G2 and 58 G2 sarcomas. Ki-67 labelling index (LI) was calculated from whole block sections for the possibility to select the most proliferative regions. The LI ranged from 1.26 to 75.5% (median 26.7%) and strongly correlated with the mitotic count (p rs for adult patients with STSs and may assist in establishment of the histological grade in STSs.