2009
Effect of Cadmium Chloride on Metallothionein Levels in Carp
KOVAROVA, Jana, Rene KIZEK, Vojtech ADAM, Danka HARUŠTIAKOVÁ, Olga CELECHOVSKA et. al.Základní údaje
Originální název
Effect of Cadmium Chloride on Metallothionein Levels in Carp
Název česky
Effect of Cadmium Chloride on Metallothionein Levels in Carp
Autoři
KOVAROVA, Jana (203 Česká republika), Rene KIZEK (203 Česká republika), Vojtech ADAM (203 Česká republika), Danka HARUŠTIAKOVÁ (703 Slovensko, garant), Olga CELECHOVSKA (203 Česká republika) a Zdenka SVOBODOVA (203 Česká republika)
Vydání
Sensors, Basel, Switzerland, Molecular Diversity Preservation Interna, 2009, 1424-8220
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10511 Environmental sciences
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.821
Kód RIV
RIV/00216224:14310/09:00035776
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000267339700044
Klíčová slova česky
heavy metals; bioaccumulation; Cyprinus carpio
Klíčová slova anglicky
heavy metals; bioaccumulation; Cyprinus carpio
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 23. 2. 2010 21:17, RNDr. Danka Haruštiaková, Ph.D.
V originále
Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.
Česky
Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.
Návaznosti
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